表面增强拉曼光谱技术在农药福美双检测中的应用

Sensitive surface-enhanced Raman spectroscopy detection of thiram based on hybrid substrate

目的制备具有表面增强拉曼光谱(surface-enhanced Raman spectroscopy,SERS)性能的复合基底并用于农药福美双的检测。方法通过沉积法制备具有粗糙金属表面的金基底,经氨基化处理后与银溶胶结合,形成金基底/银纳米粒子复合基底。以对巯基苯胺(PATP)为探针分子,比较复合基底和金基底拉曼强度。结果与结论与金基底相比,复合基底对PATP具有更强的拉曼增强效果。复合基底用于检测农药福美双,其检测灵敏度可达10-9mol/L。应用金基底/银纳米粒子复合基底的SERS可定量检测福美双。

Objective To prepare hybrid substrate and apply it to detect thiram with surface-enhanced Raman spectroscopy（SERS） which provides unique molecular vibration information.Methods The Au substrate was prepared by deposition of gold film on the silver substrate that had a rough surface.The Au substrate was treated with amination as a linker with the silver sol before the hybrid substrate was formed.With PATP as a probe molecule,the Raman intensity of PATP on the Au substrate and the hybrid substrate was compared,respectively.Results and Conclusion PATP had stronger Raman intensity on hybrid substrate than on the Au,and the detection limit was 10-9mol / L.This method can be used for quantitative detection on the hybrid substrate by SERS.