萘醌溶液的吸收和荧光光谱分析

Absorption and fluorescence spectra analysis on naphthoquinone

研究了萘醌的吸收光谱特性和紫外光激励下的荧光光谱特性,分析了吸收光谱和荧光光谱随萘醌浓度改变的变化规律及两种光谱技术检测萘醌的工作曲线和检测限。结果显示,萘醌溶液对波长245 nm的紫外光有强烈的吸收,在331 nm处有次吸收峰出现,且吸收峰的强度随溶液浓度的降低（10→2μM）呈线性衰减。萘醌吸收光后有较好的荧光发射性能,在250-600 nm波段有明显的荧光发射,荧光峰位于423 nm处。最佳激励波长为306 nm,荧光峰的强度随溶液浓度的变化（10→2μM）呈线性分布。萘醌浓度梯度的吸收和荧光光谱分析结果显示,两种检测方法对于萘醌的检测限均为2μM。

The characteristics of absorption and fluorescence spectra,as well as the concentration-dependent spectra of naphthoquinone are studied. The results illustrate that the naphthoquinone has a strong absorption peak at 245 nm and a weak peak at 331 nm,and a linear calibration curve is found between the absorption peak intensity at 245 nm and the concentration from 10 μM to 2 μM. The naphthoquinone exhibits an obvious fluorescence emission with a peak at 423 nm,with the optimum excitation wavelength at306 nm. The concentration-dependent fluorescence spectra show a linear relationship from 10 μM to 2μM. Both the results of absorption and fluorescence investigations for naphthoquinone reveal that the limit of detection（ LOD） is 2 μM.