芪红脑苏汤对急性不完全性脑缺血模型大鼠的脑保护作用

Protective effect of Qihongnaosu-decoction on acute incomplete cerebral ischemia in rats

目的探讨芪红脑苏汤对急性不完全性脑缺血模型大鼠的脑保护作用。方法 48只wistar大鼠随机分为6组予以处理,每组各8只,分别为假手术组、急性不完全性脑缺血模型组、尼莫地平对照组(8×10-3g/kg)和芪红脑苏汤高(31.4g/kg)、中(15.7 g/kg)、低(7.9 g/kg)剂量组。以10 ml/kg给药容积灌胃给药,每天1次,共给药7天。第7天给药1 h后,用20%乌拉坦(1.0 g/kg,ip)麻醉动物,分离双侧颈总动脉,引入双线分别结扎,形成急性实验性不完全性脑缺血模型,假手术组不结扎。观察各组大鼠脑含水量、脑指数及脑血管通透性。结果急性不完全性脑缺血模型组大鼠脑指数、脑含水量和脑组织中伊文斯兰含量均增高,经芪红脑苏汤治疗后均不同程度降低。结论芪红脑苏汤对脑缺血大鼠具有一定的保护作用。

Objective To investigate the protective effect of Qihongnaosu-decoction on acute incomplete cerebral ischemia.Methods Forty-eight male Wistar rats were randomly divided into 6 groups： sham-operated group,acute incomplete cerebral ischemia model group,Nimodipine group（ 8×10^-3g / kg）,and Qihongnaosu-decoction of high（ 31. 4 g / kg）,medium（ 15. 7 g / kg） and low dose（ 7. 9 g/kg） groups. The acute incomplete cerebral ischemia rat model was established by permanently ligaturing the common carotid arteries on both sides of the rats. All groups were given corresponding drug once a day by oral gavages for 7 days. The changes of cerebral water content,cerebral index and the Evans Blue exudation from brain capillaries were observed. Results The cerebral water content,cerebral index and Evans Blue exudation in the acute incomplete cerebral ischemia model group were increased. Qihongnaosu-decoction could alleviate the injury of acute incomplete cerebral ischemia to different degree. Conclusion Qihongnaosu-decoction may have a protective effect on acute incomplete cerebral ischemia injury.