HPLC法同时测定艾附暖宫丸中芍药苷、阿魏酸和川续断皂苷Ⅵ

Determination of paeoniflorin,ferulic acid and asperosaponin Ⅵ in Aifu Nuangong Pills by HPLC

目的建立同时测定艾附暖宫丸中芍药苷、阿魏酸、川续断皂苷Ⅵ的高效液相色谱法。方法艾附暖宫丸50%甲醇提取液的高效液相色谱分析以C18色谱柱为固定相,以乙腈-0.1%磷酸溶液为流动相,体积流量1mL/min,柱温为30℃,线性梯度洗脱,芍药苷、川续断皂苷Ⅵ检测波长为230nm、阿魏酸检测波长为321nm。结果芍药苷在0.502-5.016 7μg（r=0.999 3）范围内、阿魏酸在0.147-1.760μg（r=0.999 8）范围内、川续断皂苷Ⅵ在0.975-11.480μg（r=0.999 5）范围内,对照品进样质量和色谱峰峰面积呈良好线性关系,加样回收率分别为97.8%、100.5%和97.6%,RSD值分别为2.0%、2.0%和1.8%。结论建立的高效液相色谱法简便稳定、准确可靠,可用于艾附暖宫丸的质量控制。

AIM To set up an HPLC method for determining three bioactive components,paeoniflorin,ferulic acid and asperosaponin Ⅵ in Aifu Nuangong Pills.METHODS The chromatographic separation of methanolic extract of Aifu Nuangong Pills was performed on Global-C18（ 250 mm × 4.6 mm,5 μm） column in a gradient elution manner using a mixture of acetonitrile and 0.1% phosphoric as mobile phase.The flow rate was 1 m L / min and column temperature was maintained at 30 ℃.The detection wavelength of paeoniflorin and asperosaponinⅥ was set up at 230 nm,ferulic acid at 321 nm.RESULTS The linear range was 0.502- 5.016 7 μg（ r = 0.999 3） for paeoniflorin,0.147- 1.760 μg（ r = 0.999 8） for ferulic acid and 0.975- 11.480 μg（ r = 0.999 5） for asperosaponin Ⅵ.The average recoveries were 97.8% for paeoniflorin with RSD = 2.0%,100.5% for ferulic acid with RSD = 2.0%,and 97.6% for asperosaponinⅥ with RSD = 1.8%,respectively.CONCLUSION The method is simple and convenient,reliable and accurate,it can be utilized as a quality control method for Aifu Nuanging Pills.