摘要
目的通过高效液相色谱-电喷雾质谱(HPLC-ESI-MS/MS)联用技术,对菥蓂中的主要黄酮成分进行分析鉴定。方法菥蓂水提醇沉物的分离采用Thermo Scientific Syncronis C18色谱柱(150mm×2.1mm,3μm),以0.1%甲酸水(A)-乙睛(B)为流动相梯度洗脱进行色谱分离,体积流量0.35 m L/min,检测波长为254nm;电喷雾质谱在线检测,负离子模式下采集数据。结果共分析鉴定出15个黄酮类化合物。结论 9个黄酮类化合物在该植物中首次发现,即异牡荆素草酸酯、异牡荆素-7-O-葡萄糖苷、异牡荆素7,2″-二-O-β-吡喃葡萄糖苷、异荭草素、异牡荆素碳酸酯、当药黄素、当药黄素碳酸酯、木犀草素7-硫酸酯和芹菜素7-硫酸酯。
AIM To identify the main constituents of Thlaspi arvense L.by high performance liquid chromatography- electrospray mass spectrometry（ HPLC-ESI-MS / MS） detection.METHODS The separation of water extraction and ethanol sedimentation from T.arvense L.was performed on UPLC Thermo Scientific Syncronis C18column（ 150mm × 2.1mm,1.3μm） with a mobile phase using water containing 0.1% formic acid（ A） and acetonitrile（ B） in a gradient elution manner.The data collection was adopted in negative ion mode using electron spray with ESI source.RESULTS Fifteen flavonoids were well separated and identified from the extracts of T.arvense L.in all.CONCLUSION Nine flavonoids are identified for the first time,namely isovitexin ethanedioate,isovitexin-7-O-glucoside,isovitexin 7,2″-di-O-glucoside,isoorientin,isovitexin carbonate,swertisin,swertisin carbonate,luteolin 7-sulfate and apigenin 7-sulfate
出处
《中成药》
CAS
CSCD
北大核心
2015年第3期556-561,共6页
Chinese Traditional Patent Medicine
基金
“重大新药创制”科技重大专项(2010ZX09401-306-3-15)
