摘要
研究白刺叶在不同生长时期内黄酮提取物的抗氧化及抑菌活性,为白刺的资源开发利用提供新的途径。试验采用紫外分光光度法测定在不同生长时期内白刺提取物中总黄酮类物质的含量,得率分别为0.654%,1.17%和0.53%。采用3种不同的方法 (DPPH,羟基自由基,ABT S+)评价了白刺黄酮提取物的抗氧化活性,采用牛津杯法测定抗菌活性,采用二倍稀释法测定最低抑菌浓度。结果表明,在不同生长时期内的白刺叶总黄酮提取物在相同的质量浓度下,表现出的抗氧化活性不同,对DPP H,羟基自由基,ABT S+均具有一定的清除作用。白刺叶总黄酮提取物对DPPH的最大清除率:展叶期(92.5%)>花期(88.9%)>发芽期(56.5%),对羟基自由基的最大清除率:展叶期(97.3%)>花期(94.1%)>发芽期(90.3%),对ABTS+的最大清除率:花期(86.5%)>发芽期(57.6%)>展叶期(39.3%)。不同生长时期内的白刺叶总黄酮提取物对大肠杆菌和金黄色葡萄球菌均具有较强的抑制作用,在展叶期的总黄酮提取物对大肠杆菌和金黄色葡萄球菌的抑制作用最佳,为21.85 mm和20.4 mm。说明在不同生长时期内的白刺叶总黄酮提取物可以作为一种天然的抗氧化剂和抑菌剂而广泛利用。
The antioxidation and antibacterial properties of flavonoids extracted from Nitraria leaves were studied,which would provide valuable information for the development of Nitraria.Using ultraviolet spectrophotometry method,the content of the obtained total flavonoids was determined to be 0.654%,1.17% and 0.53%,respectively.Flavonoids extracted from Nitraria leaves possessed strong capability of eliminating DPPH,·OH,ABTS^＋.The antibacterial activity was evaluated by Oxford cup method,using the double dilution method determination of minimal inhibitory concentrations（mic）.The results showed that under the same mass concentration,the flavonoids extracts from Nitraria leaves in different growth period,show different antioxidant activity on DPPH,hydroxyl radical,ABTS^＋.The flavonoids extracts from Nitraria leaves against the activity of DPPH,·OH,ABTS^＋ were leaf expansion（92.5%）〉 florescence（88.9%）〉 germination（56.5%）,leaf expansion（97.3%） 〉florescence（94.1%）〉 germination（90.3%）,florescence（86.5%） 〉germination（57.6%）〉 leaf expansion（39.3%）,respectively.The flavonoids of Nitraria leaves in different growth period had a certain bacteriostatic effect to Escherichia coli,staphylococcus aureus but that of in leaf expansion inhibition capability（21.85 mm,20.4 mm） was the strongest.It is indicated that the flavonoids extracts from Nitraria leaves can be used as a natural antioxidant and antibacterial agent and extensive use.
出处
《食品工业》
北大核心
2015年第3期94-98,共5页
The Food Industry
