摘要
制备了二氧化硅包裹的具有"金属-有机物-金属"三明治结构的表面增强拉曼散射(SERS)光学免疫检测探针,研究了探针的形貌、结构、组分等多种因素对其SERS特性的影响,探索出具有较好SERS活性的制备参数。之后利用探针上集成的SERS信号,采用三明治免疫法,实现了激酶抑制因子(p21)和人体抑癌基因(p53)两种疾病因子的高特异性检测。
A novel kind of silica coated‘metal-organic-metal ＇sandwich-type surface enhanced Raman scattering（ SERS） nanoprobe has been successfully prepared. To explore the optimal preparation condition,the influences of morphology,structure and composition on the SERS performance of the nanoprobe were carefully investigated. After successful fabrication of the nanoprobe,its application in the detection of two biomarkers（ p21 and p53） was investigated by using a sandwich-type immunoassay protocol. The results confirmed that the nanoprobe can generate strong SERS signals and can distinguish p21 and p53 with a good specificity.
出处
《电子器件》
CAS
北大核心
2015年第1期1-6,共6页
Chinese Journal of Electron Devices
基金
国家大学生创新训练计划项目(1310286028)
关键词
表面增强拉曼散射
光学探针
三明治结构
金属纳米粒子
免疫检测
surface enhanced Raman scattering
optical nanoprobe
sandwich structure
metal nanoparticles
immunoassay
