转GDNF基因的BMSCs移植对大鼠脑出血突触可塑性的影响

Effects of GDNF-transfected BMSCs on synaptic plasticity in rats with intracerebral hemorrhage

目的:探讨转胶质细胞源性神经营养因子基因的骨髓基质干细胞移植到脑出血大鼠后对其突触可塑性的影响。方法:采用胶原酶诱导大鼠尾壳核出血模型,于建模后第3 d在脑出血部位分别注射生理盐水、BMSCs和GDNF/BMSCs悬液20μl。将48只模型大鼠随机分为生理盐水组、BMSCs组和GDNF/BMSCs组,各组又根据取材时间不同分为1周组和2周组,每亚组8只。通过免疫组织化学与RT-PCR观察出血灶周边突触素Syn和生长相关蛋白GAP-43的蛋白与基因的表达。结果:在1周和2周两个时间点,GDNF/BMSCs组与BMSCs和生理盐水组相比Syn和GAP-43的m RNA以及免疫阳性产物显著增加。结论:GDNF/BMSCs移植较单纯BMSCs更能促进脑出血周边区域神经再生修复。

Objective： To transplant bone marrow stromal cells（BMSCs） transfected with glial cell line derived neurotrophic factor（GDNF） into rats with intracerebral hemorrhage（ICH） and investigate the effects of GDNFtransfected BMSCs on synaptic plasticity in rats with ICH. Methods： The rat models of ICH were established by injection of collagenase. Forty-eight rats were randomly divided into saline group, BMSCs group and GDNF/BMSCs group. The 20 μl of saline, suspension of BMSCs, GDNF/BMSCs were respectively injected in the three groups at the third day after operation. Each group was subdivided into subgroups of one week and two week according to the sampling time. Immunohistochemistry and RT-PCR were used to detect the expressions of proteins and transcription of genes of Syn and GAP-43 in the margin of ICH. Results： The expression of m RNA and immunoreactive products of Syn and GAP-43 in GDNF/BMSCs group or BMSCs group significantly increased more significanly than that in the saline group sampling at one week and two week respectively. Conclusion：Transplantation of BMSCs transfected by GDNF gene promotes neuranagenesis better than pure BMSCs in treatment of ICH.