期刊文献+

[Co(phen)_3]^(3+)和SWCNTs促进钌(Ⅱ)配合物对牛血清蛋白的电催化氧化 被引量:2

Electrocatalytic Oxidation of Bovine Serum Albumin Mediated by Ruthenium( Ⅱ) Complexes upon Incorporation of [Co( phen)_3]^(3+) and SWCNTs
下载PDF
导出
摘要 应用循环伏安法、微分脉冲伏安法、荧光光谱法和扫描电镜等方法研究了[Co(phen)3]3+(phen=1,10-邻菲咯啉)和单壁碳纳米管(SWCNTs)促进[Ru(bpy)2(tatp)]2+(bpy=2,2'-联吡啶,tatp=1,4,8,9-四氮三联苯)在铟锡氧化物(ITO)电极上对牛血清蛋白(BSA)的电催化氧化.结果表明,[Ru(bpy)2(tatp)]2+、BSA、[Co(phen)3]3+和SWCNTs间相互作用明显,BSA-SWCNTs的加入促进了[Ru(bpy)2(tatp)]2+在ITO上的电化学组装,[Co(phen)3]3+的加入则促进了组装到ITO电极上的[Ru(bpy)2(tatp)]2+对BSA的电催化氧化,在连续微分脉冲伏安图上呈现一个类似于色氨酸电催化氧化的特征氧化峰.通过改变离子强度、Ru(Ⅱ)或Co(Ⅲ)配合物配体结构和BSA浓度的研究发现,增强BSA与双金属键合剂间作用的因素有利于Co(Ⅲ)配合物诱导Ru(Ⅱ)配合物对BSA的电催化氧化.此外,BSA的电催化氧化响应在0.3~1.5μmol/L BSA浓度区呈线性增加变化,Co(Ⅲ)配合物的伏安响应呈线性减小.研究结果有助于理解双金属键合剂对蛋白质的电催化氧化作用,为蛋白质电化学传感器的构建提供强有力的依据. [Ru( bpy)2( tatp) ]2 +( bpy = 2,2'-bipyridine and tatp = 1,4,8,9-tetra-aza-triphenylene),single-walled carbon nanotubes( SWCNTs) and [Co(phen)3]^3+( phen = 1,10-phenanthroline) are successfully used to promote the electrochemical oxidation of bovine serum albumin( BSA) on an indium-tin oxide( ITO) electrode by means of cyclic voltammetry,differential pulse voltammetry,fluorescence spectroscopy and scanning electron microscope.There are obvious interactions among [Ru( bpy)2( tatp) ]2 +,BSA,[Co(phen)3]^3+and SWCNTs. In the present protocol,the addition of BSA-SWCNTs is found to promote the electrochemical assembly of [Ru( bpy)2( tatp) ]3 +on the ITO electrode,and the simultaneous presence of [Co(phen)3]^3+and SWCNTs facilitates the electrocatalytic oxidation of BSA mediated by [Ru( bpy)2( tatp) ]2 +assembled on the ITO surface. BSA shows a characteristic oxidation peak,which is similar to that of tryptophan( Trp) under our experimental conditions. The evidences from the effects of ionic strength,the structure of Ru( Ⅱ) and Co( Ⅲ) complexes and BSA concentration suggest that the strong binding interaction of Ru( Ⅱ) and Co( Ⅲ) complexes to BSA facilitates the electrocatalytic oxidation of BSA. In addition,the electrocatalytic oxidation response of BSA shows a linear increase in the range from 0. 3 to1. 5 μmol / L,and the Co( Ⅲ)-based voltammetric current exhibits a linear decrease. The present study helps us understand the electrocatalytic oxidation of proteins mediated by double metal binding agents,as well as provides asignificant basis for protein electrochemical sensor.
出处 《华南师范大学学报(自然科学版)》 CAS 北大核心 2015年第2期32-38,共7页 Journal of South China Normal University(Natural Science Edition)
基金 国家自然科学基金项目(21271075) 广东省自然科学基金项目(10351063101000001 S2012010008763)
关键词 牛血清蛋白 钌(Ⅱ)配合物 钴(Ⅲ)配合物 碳纳米管 电催化氧化 bovine serum albumin Ruthenium(Ⅱ) complex Cobalt(Ⅲ) complex carbon nanotube electrocat-alytic oxidation
  • 相关文献

参考文献25

  • 1张会娜,郭智勇,盖盼盼.蛋白膜伏安法研究进展[J].分析化学,2009,37(3):461-465. 被引量:3
  • 2刘慧宏,庞代文.氧化还原蛋白质电化学研究[J].化学进展,2002,14(6):425-432. 被引量:19
  • 3Hirsch R, Katz E, Willner I. Magneto-switchable bioel- ectrocatalysis[J]. Journal of the American Chemical So- Ciety, 2000, 122(48) :12053 - 12054.
  • 4胡乃非,李溱,马红艳.肌红蛋白在双十二烷基二甲基溴化铵-粘土多双层复合薄膜电极上的电化学与电催化[J].高等学校化学学报,2001,22(3):450-454. 被引量:15
  • 5Liu G Z, Gooding J J. An interface comprising molecular wires and poly (ethylene glycol) spacer units self-assem- bled on carbon electrodes for studies of protein electro- chemistry [ J ]. Langmuir, 2006, 22 ( 17 ) :7421 - 7430.
  • 6Hu Y J, Liu Y, Zhang L X,et al. Studies of interaction between eolchicine and bovine serum albumin by fluores- cence quenching method[ J]. Journal of Molecular Struc- ture, 2005, 750( 1 ) :174 - 178.
  • 7Ghosh S, Jana S, Nath D, et al. Fluorescent probing of protein bovine serum albumin stability and denaturation u- sing polarity sensitive spectral response of a charge trans- fer probe [ J ]. Journal of Fluorescence, 2011, 21 ( 1 ) : 365 - 374.
  • 8Chen H J, Zhang Z H, Luo L J, et al. Surface-imprinted chitosan-coated magnetic nanopartieles modified multi- walled carbon nanotubes biosensor for detection of bovine serum albumin[ J]. Sensors and Actuators B: Chemical, 2012, 163(1) :76 -83.
  • 9Hamilton A, Breslin C B. The incorporation of bovine se- rum albumin into a polypyrrole film in one simple step [J]. ECS Transactions, 2014, 58(46) :1 -13.
  • 10Ji S B, Yan Z H, Wu J W, et al. One-step electrochemi- cally co-assembled redox-active [ Ru ( bpy ) 2 ( tatp ) ] 2 +-BSA-SWCNTs hybrid film for non-redox protein biosen- sors[ J]. Biosensors and Bioelectronics, 2013, 39 (1) : 106 - 111.

二级参考文献55

  • 1周波,孙润光,王丽华,宋世平,樊春海.蛋白质直接电化学研究及其应用[J].化学进展,2006,18(7):1009-1013. 被引量:7
  • 2Leger C, Lederer F, Guigliarelli B, Bertrand P. J. Am. Chem. Soc. , 2006, 128(1) : 180 - 187
  • 3Heering H A, Wiertz F G M, Dekker C, Vries S D. J. Am. Chem. Soc. , 2004, 126(35): 11103-11112
  • 4Aguey-Zinsou K F, Bernhardt P V, Leimk hler S. J. Am. Chem. Soc. , 2003, 125(50) : 15352 - 15358
  • 5Hudson J M, Heffron K, Kotlyar V, Sher Y, Maklashina E, Cecchini G, Armstrong F A, J. Am. Chem. Soc. , 2005, 127(19) : 6977 -6989
  • 6Hirst J, Armstrong F A. Anal. Chem. , 1998, 70(23) : 5062 -5071
  • 7Leger C, Elliott S J, Hoke K R, Jeuken L J C, Jones A K, Armstrong F A. Biochemistry, 2003, 42(29) : 8653 -8662
  • 8Hirst J, Jameson G N L, Allen J W A, Armstrong F A. J. Ant. Chem. Soc. , 1998, 120(46) : 11994 - 11999
  • 9Jeuken L J C, Wisson L J, Armstrong F A. Inorg. Chim. Acta, 2002, 331 : 216 -223
  • 10Hoeben F J M, Heller I, Albracht S P J, Dekker C, Lemay S G, Heering H A. Langmuir, 2008, 24(11): 5925 -5931

共引文献33

同被引文献30

  • 1Hirsch R, Katz E, Willner I. Magneto-switchable bioel-ectrocatalysis [J]. Journal of the American Chemical So-ciety, 2000, 122:12053 -12054.
  • 2Chakraborty A, Seth D,Setua P,et al. Photoinduced e-lectron transfer in a protein-surfactant complex : Probingthe interaction of SDS with BSA[ J]. Journal of PhysicalChemistry B, 2006,110: 16607 -16617.
  • 3Wang J, Liu G,Jan M R. Ultrasensitive electrical bio-sensing of proteins and DNA : Carbon-nanotube derivedamplification of the recognition and transduction events[J]. Journal of the American Chemical Society,2004,126:3010 -3011.
  • 4Shleev S,Tkac J, Christenson A, et al. Direct electrontransfer between copper-containing proteins and electrodes[J ]. Biosensors & Bioelectronics, 2005,20:2517 -2554.
  • 5Liu G, Gooding J J. An interface comprising molecularwires and poly ( ethylene glycol) spacer units self-assem-bled on carbon electrodes for studies of protein electro-chemistry[ J]. Langmuir, 2006,22:7421 -7430.
  • 6Sano Y, Tanaka I. Detection of differentially expressedvariant histone H3. 3 in the vegetative nucleus of lily pol-len[ J]. Sexual Plant Reproduclion, 2007,20;27 -33.
  • 7Cai H, Cao X, Jiang Y,et al. Carbon nanotube-en-hanced electrochemical DNA biosensor for DNA hybrid-ization detection[ J]. Analytical and Bioanalytical Chem-istry, 2003, 375:287 -293.
  • 8Wang J. Electrochemical biosensors: Towards point-of-care cancer diagnostics[ J]. Biosensors & Bioelectronics,2006, 21:1887 -1892.
  • 9Yang I V,Ropp P A, Thorp H H. Toward electrochemi-cal resolution of two genes on one electrode ; Using 7-dea-za analogues of guanine and adenine to prepare PCR prod-ucts with different [ J ]. Analytical Chemistry, 2002,74:347 -354.
  • 10Wu H W, Liu S C, Zhu W P, et al. A sensitive electro-chemical biosensor for detection of histone deacetylase ac-tivity using an acetylated peptide [ J ]. Electroanalysis,2012,24:2365 -2370.

引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部