复州牛超数排卵效果研究

Study on Superovulation Effect in Chinese Fuzhou Cattle

为了建立一个合理有效的复州牛超数排卵方案,获得更多更优的胚胎,长期保存复州牛的基因,试验从不同批次、哺乳情况、不同来源促卵泡素(FSH)、不同FSH剂量等方面分析了47头复州牛超数排卵效果。结果表明,经超数排卵处理的复州牛头均回收卵数7.98枚,其中头均有效胚数4.62枚;供体牛体况好、超数排卵方案细化的第3批次效果最好,头均有效胚数8.25枚,显著高于第1批次(2.00枚)和第2批次(2.79枚)(P<0.05);未哺乳牛头均黄体数、头均回收卵数和头均有效胚数分别为15.38个、7.63枚、5.75枚,极显著高于哺乳牛的10.64个、2.18枚、0.64枚(P<0.01);日本产FSH头均回收卵数、头均有效胚数分别为12.70和7.50枚,动物所产FSH分别为10.62和7.38枚,二者间差异不显著(P>0.05);动物所产FSH的适宜剂量为每头8.8mg,头均有效胚数7.38枚,有效胚率为69%。通过优化复州牛的超数排卵方案,取得了理想的超数排卵效果。

To build a reasonable and valid superovulation scheme,get more superior embryos and conserve the whole genome of Chinese native Fuzhou cattle,47 Fuzhou donor cows were hired to perform superovulation treatment in the present study.The superovulation effects were systemically analyzed and compared from different batch processing of superovulation,different lactation status,different brands and dosages of FSH.The results showed that via superovulating,7.98 recycled eggs per head were collected in the Fuzhou donor cows,of which effective embryos were4.62.The average number of effective embryos in the third group（8.25）was significantly higher than that in the first（2.00）and the second（2.79）groups（P0.05）.The average number of luteums（15.38）,the average number of recycled eggs（7.63）and the average number of effective embryos（5.75）of the cattle not in milk were extremely significantly higher than that of the cattle in milk（10.64,2.18 and 0.64,respectively）（P0.01）.The average number of recycled eggs and the average number of effective embryos of Fuzhou cattle treated with Jp-FSH were 12.70 and 7.50,respectively.The similar superovulation effects were observed in the Fuzhou cattle treated with Ch-FSH（10.62 and 7.38,respectively）（P0.05）.The suitable dose of Ch-FSH was8.8mg per head,The average number of effective embryos and the ratio of effective embryos were7.38 and 69%,respectively.The results indicated that desired superovulation effects were obtained through optimizing the superovulation schemes in Fuzhou cattle.