曲靖地区仔猪腹泻细菌性病原的分离鉴定与耐药性分析

Isolation,Identification and Drug Resistance Analysis of Bacterial Pathogen of Piglets Diarrhea Syndrome in Qujing Area

为了解曲靖地区仔猪出现腹泻的细菌性病因,对5个养殖场148份出现仔猪腹泻症状的样品进行细菌分离培养及耐药性分析,结果显示,检出致病性大肠杆菌(E.coli)114份,阳性率为77.03%;沙门氏菌(Salmonella)26份,阳性率为17.57%,混合感染率为17.57%。致病性大肠杆菌和沙门氏菌对多种抗生素产生了耐药性,其中114株致病性大肠杆菌中97株呈多重耐药性,占总菌数的85.09%,26株沙门氏菌中20株呈多重耐药性,占总菌数的76.92%。结果表明,致病性大肠杆菌是导致当地哺乳仔猪腹泻的主要细菌性病原,部分仔猪继发感染沙门氏菌,两种细菌对多种抗生素产生了耐药性,多重耐药现象较严重。

In order to know the bacterial causes of piglet diarrhea in the Qujing area,the study collected 148 samples from 5farms appearing diarrhea symptoms for bacterial isolation,culture and drug resistance analysis.The results showed that the detection of pathogenic E.coli was 114 strains,the positive rate was 77.03%;Salmonella was 26 strains,the positive rate was 17.57%,mixed infection rate was 17.57%.Pathogenic E.coli and Salmonella were resistant to a variety of clinic medicines.The 97 strains were multidrug resistance in 114 pathogenic E.coli,showing85.09% of the total number of bacteria;20of 26 Salmonella were multidrug resistant,showing76.92% of the total number of bacteria.The results suggested that pathogenic E.coli was major bacterial pathogen which causing piglets diarrhea in Qujing local area,some piglets were secondary infected with Salmonella,two kinds of bacteria had both appeared high resistance,multidrug resistance were more serious.