加味四君子汤对脾虚小鼠免疫机能、抗应激和消化吸收的影响

Effect of Supplemantary Sijunzi Decoction on Immune Function,Anti-stress,Digestion and Absorption of Spleen Deficiency Mice

为研究加味四君子汤的药效学,试验通过灌服大黄水煎液制备小鼠脾虚症模型,观察不同剂量加味四君子汤对脾虚小鼠一般体征、免疫机能、抗应激能力、胃肠运动和吸收功能的影响。结果表明,加味四君子汤中,高剂量组可改善脾虚小鼠的体征,显著增加小鼠体重和腹腔巨噬细胞吞噬率(P<0.05),在一定程度上提高小鼠体温;中剂量组可显著提高血清中IgM含量、脾脏指数和胸腺指数(P<0.05);不同剂量加味四君子汤均能显著提高腹腔巨噬细胞吞噬指数和延长小鼠游泳持续时间(P<0.05),改善常压耐缺氧能力,降低胃排空率,减缓小肠的推进,提高脾虚小鼠尿液D-木糖排泄量和血清淀粉酶活性。综上所述,不同剂量的加味四君子汤能改善小鼠脾虚症状,提高小鼠的免疫机能、抗应激和消化吸收能力。

This experiment was conducted to study pharmacodynamics of supplemantary sijunzi decoction（SJZD）.In order to evaluate the effect of SJZD on the general signs,immune function,anti-stress,gastrointestinal motility and absorption of spleen deficiency mice,the model of spleen deficiency was prepared with rheum officinale solution.The results showed that the general signs were improved,body weight,body temperature of spleen deficiency mice and devouring percentage of macrophage cell were significantly increased in high dosage-treated groups（P0.05）.The content of serum IgM,spleen and thyrus index were singficantly increased in medium dosage-treated group（P0.05）.The devouring index of macrophage cell and swimming duration were significantly increased in various dosage of supplemantary SJZD（P0.05）,anti-hypoxia ability could be prolonged,gastric emptying rate and relative percentage each small intestine propulsion were decreased in various dosage of SJZD,D-xylose excretion contents and serum amylase activities were enhanced in various dosage of supplemantary SJZD.In conclusion,SJZD could improve the spleen deficiency symptoms,enhance the immune function,anti-stress ability and gastrointes-tinal motility.