急性死亡猪中猪丹毒杆菌的分离鉴定

Isolation and Identification of Swine Erysipellthrix rhusiopathiae in Acute Cases

本试验从江西省南昌市某规模化猪场送检的2份疑似猪丹毒杆菌引起的急性死亡猪组织中分离出2株可疑菌株,经细菌分离培养、染色镜检、生化试验等实验室诊断,进一步以猪丹毒杆菌16SrRNA的特异性引物进行PCR鉴定,确定2株分离菌为猪丹毒杆菌。然后进行动物致病性试验及药敏试验,并对该厂常用的消毒药进行最小抑菌浓度(MIC)的测定。结果表明,1×108 CFU的剂量可致死小白鼠;分离菌对β-内酰胺类抗菌药高度敏感,对多数抗菌药耐药;该厂常用消毒药的抑菌作用已明显下降。本试验结果对临床防制猪丹毒具有参考意义。

Two strains of swine Erysipellthrix rhusiopathiae,which were characterized by cultural character,shape observation,biochemical test,polymerase chain reaction detection,animal pathogenicity experiment,antibiotic sensitivity test and MIC determination,were isolated from infected pig farms in Jiangxi province.The animal pathogenicity experiment demonstrated that 1×108CFU could be lethal to mice.The antibiotic sensitivity test result showed that the two strains were highly sensitive toβ-lactam antibiotics,but resistant to most of the other antibiotics.The MIC test displayed that the disinfectants used in this farm were not effective anymore.This result was important for clinic to prevent and treat swine erysipelas.