摘要
通过凯氏定氮法测定盐肤木种子总蛋白质含量,然后采用3种不同的方法提取其中的蛋白质。结果表明:盐肤木种子总蛋白质的含量约为9.501%。在本实验条件下,改良丙酮沉降法提取的蛋白质含量较TCA-丙酮提取法和碱式提取法高。正交试验确定改良丙酮沉降法较优工艺条件为:"料液比为1∶12g·mL-1,提取时间为3h,提取液与沉降蛋白所用丙酮量体积比1∶2,沉降时间为1.5h。在较优提取工艺条件下,盐肤木蛋白质的提取率达到58.44%。
The content of protein from Rhus Chnensis seed was assayed by Kjeldahl determination and three pro- tein extraction methods were employed. The results showed that the content of Rhus Chnensis seed protein was about 9. 501%. Protein extracted by the improved acetone sedimentation method was higher than by the TCA --acetone extraction and basic extraction method. The optimum process conditions of improved acetone sedi- mentation method by orthogonal test were： the ratio of solid to liquid 1 ： 12 g · mL^-1 ,extraction time 3 h,the ratio of protein extraction solution to acetone 1 ： 2,sedimentation time 1.5 h. Practicing this optimal condition, the extraction rate of Rhus Chinensis protein reached 58.44%. The experiment data was referred for further study of the Rhus Chinensis seed protein.
出处
《宿州学院学报》
2015年第2期109-112,共4页
Journal of Suzhou University
基金
国家公益性林业科研专项项目"盐肤木新品种选育与示范"(201204312)
国家自然科学基金"基于近红外与电子鼻技术的药用菊花道地药材模式识别研究"(30901972)
安徽高校省级自然科学研究重点项目"新木本油料植物盐肤木油脂资源开发应用研究"(KJ2011A273)"生姜病毒检测试剂盒的研制与生姜脱毒快繁"(KJ2013A264)
安徽省自然科学一般项目"复合酶法制备纯天然速溶绿茶的研究"(KJ2013B341)
关键词
盐肤木
蛋白质
凯氏定氮法
提取
正交试验
Rhus Chinensis
protein
Kjeldahl determination
extraction
orthogonal test
