低强度激光对软骨细胞增殖的生物刺激作用

Biostimulation of low-level laser on the proliferation of chondrocytes

八周龄健康SD大鼠的后肢胫骨平台分离培养软骨细胞,用噻唑蓝(MTT)法检测细胞活性,研究了658、785和830nm 3种波长低强度激光对大鼠关节软骨细胞增殖的影响。结果显示,在胎牛血清体积分数为2%和5%的培养条件下,5J·cm-2的658nm激光能明显促进软骨细胞增殖;658、785和830nm 3种波长低强度激光均能促进软骨细胞增殖,但是具有波长差异性和光剂量相关性。结果表明,低强度激光辐照具有在体治疗软骨损伤的潜能。

The self-healing ability of cartilage after damage is poor. Until now there is no perfect method to treat cartilage injuries in clinical field. Low-level laser therapy （LLLT）, which is a damage-free meth- od, has been known since the first laser was invented. In the present study, in order to investigate the po- tential of low-level laser irradiation in treating cartilage injuries, the effect of low-level laser irradiation （using three different low-level lasers,namely 658 nm,785 nm and 830 nm） on the proliferation of cul- tured chondrocytes in vitro was performed. The chondrocytes were isolated from the cartilages of hind legs of healthy 8-week-old SD rats and the cell proliferation was assessed by thiazolyl blue tetrazolium bromide （MTT） assay. The results show that irradiation using 658 nm laser with 5 J ~ cm-z could sig- nificantly stimulate the proliferation of chondrocytes cultured with fetal bovine serum at 2 % and 5 %. All the three of the different low-level lasers （wavelengths at 658 nm,785 nm and 830 nm） could obviously promote chondrocytes proliferation in a wavelength-different and dose-dependent manner. All the results demonstrate that low-level laser irradiation has the potential to treat cartilage injuries in vivo.