树脂吸附法分离纯化非达霉素的研究

Studies on the separation and purifi cation of fi daxomicin by resin adsorption method

目的研究大孔吸附树脂分离纯化非达霉素的工艺条件。方法以非达霉素的吸附量为考察指标,从中筛选树脂;并对吸附及解吸条件进行优化,得到适宜的纯化操作条件。结果 HZ816树脂为最佳吸附剂,优化的条件为吸附流速为2BV/h,解吸剂为80%的乙醇-水溶液。在此实验条件下所得产品纯度≥90%,收率≥60%。结论 HZ816树脂对非达霉素有较好的吸附分离性能,适合于非达霉素的提纯。

Objective To study technological parameters of fidaxomicin separation and purification with macroporous adsorption resin. Methods The adsorption capacity offidaxomicin was used as index toselect the resin; and the optimal purification process was determined by improving the adsorption and desorption conditions. Results Resin HZ816 was selected as the best adsorbent, the optimum condition was investigated as follows： adsorption flowrate of 2BV/h, 80% ethanol-H20 as eluent. Under the optimum condition the purity of the final product was over 90% and the yield of fidaxomicin was above 60%. Conclusion Resin HZ816 showed a perfect separation performance on fidaxomicin and was suitable to purify it.