小茴香挥发油纯胶包合物的制备工艺

Preparation Technology of Starch Sodium Octenyl Succinate Inclusion Compound of Volatile Oil from Foeniculi Fructus

目的：优选小茴香挥发油纯胶包合物的制备工艺并考察其稳定性。方法：采用超声细胞破碎法包合小茴香挥发油,以包合物收率和挥发油包合率的综合评分为指标,通过正交试验考察超声工作（间隙时间、包合总时间、挥发油-纯胶对挥发油包合工艺的影响,通过差示扫描量热法、薄层色谱和红外光谱进行结构鉴定,并考察包合物的强光照射、高温、高湿稳定性。结果：最佳包合工艺为超声工作时间15s,间隙时间20s,超声包合总时间12min,小茴香挥发油-纯胶（1∶6）,小茴香挥发油纯胶包合物已形成。在强光、高温、高湿影响下,包合物中挥发油质量分数分别下降14.5%,19.5%-38.6%,15.5%-30.0%。结论：优选的包合工艺简单可行,包合物的挥发油包合率高且稳定性好,为纯胶在挥发油包合工艺中的应用提供参考。

Objective： To optimize preparation process of starch sodium octenyl succinate（SSOS）inclusion compound of volatile oil from Foeniculi Fructus and investigate its stability.Method： Ultrasonic cell disruption was adopted to prepare SSOS inclusion compound of volatile oil from Foeniculi Fructus, taking comprehensive score of yield of inclusion compound and inclusion rate of volatile oil as index,orthogonal test was employed to optimize inclusion process with working-interval time,total inclusion time and ratio of volatile oil from Foeniculi Fructus to SSOS as factors.Differential scanning calorimetry（DSC）, thin layer chromatography（TLC） and infrared spectroscopy（IR） were used for structure characterization of inclusion compound.Stability of inclusion compound was examined under strong light, high temperature and high humidity.Result： Optimum inclusion conditions were as follows： working time of 15s,interval time of 20s,total inclusion time of 12min,ratio of volatile oil from Foeniculi Fructus to SSOS of 1 ∶ 6.SSOS inclusion compound of volatile oil from Foeniculi Fructus formed successfully.Under affecting factors,the content of volatile oil in inclusion compound decreased 14.5%,19.5%-38.6% and 15.5%-30.0%,respectively.Conclusion： This optimized inclusion process was stable and feasible with high inclusion rate,it provides a reference for application of SSOS in inclusion process of volatile oil.