HPLC-DAD测定茵陈蒿汤中儿茶素、绿原酸、西红花苷Ⅰ、西红花苷Ⅱ的含量

Determination of Catechin,Chlorogenic Acid,Crocin-Ⅰ,Crocin-Ⅱ in Yinchenhao Decoction by HPLC-DAD

目的：建立HPLC-DAD测定茵陈蒿汤中儿茶素、绿原酸、西红花苷Ⅰ、西红花苷Ⅱ含量的方法。方法：采用Symmetry C18色谱柱（4.6mm×250mm,5μm）,流动相乙腈-0.1%磷酸水梯度洗脱,流速1.0mL·min-1,柱温30℃,儿茶素检测波长202nm,绿原酸检测波长330nm,西红花苷Ⅰ,Ⅱ检测波长440nm。结果：儿茶素、绿原酸、西红花苷Ⅰ,Ⅱ分别在0.18-0.9（r=0.999 8）,0.062 4-0.312（r=0.999 9）,0.441-2.205（r=0.999 9）,0.023 52-0.117 6μg（r=0.999 9）与峰面积呈良好线性关系;加样回收率分别为98.56%（RSD 1.8%）,99.07%（RSD 1.8%）,97.41%（RSD 1.9%）和98.46%（RSD1.9%）。结论：该法简便、准确、专属性强,可用于茵陈蒿汤中4种成分的含量测定。

Objective： To establish a method for simultaneous determination of catechin,chlorogenic acid,crocin-Ⅰ,and crocin-Ⅱ in Yinchenhao decoction by HPLC-DAD.Method： The Symmetry C18 column（4.6mm × 250mm,5μm） was used,the mobile phase was acetonitrile-0.1% phosphoric acid solution with gradient elution at a flow rate of 1.0 m L·min-1.The column temperature was kept at 30 ℃ and the wavelength was set at 202nm for catechin,330nm for chlorogenic acid,and 440nm for crocin-Ⅰand crocin-Ⅱ,respectively.Result： The linear range of catechin,chlorogenic acid,crocin-Ⅰ,and crocin-Ⅱ were 0.18-0.90（r = 0.999 8）,0.020 8-0.374 4（r = 0.999 9）,0.147-2.646（r = 0.999 9） and 0.007 84-0.141 12（r = 0.999 9）μg,respectively.The mean recovery of these 4 components were 98.56%（RSD 1.8%）,99.07%（RSD 1.8%）,97.41%（RSD 1.9%）,and 98.46%（RSD 1.9%）,respectively.Conclusion： This method is simple,accurate and specific,and can be used for the determination of 4 constituents in Yinchenhao decoction.