不同大小亚贡叶化学成分比较

Comparison on Chemical Constituents from Different Size of Yacon Leaves

目的：比较不同大小亚贡叶的高效液相指纹图谱,并对亚贡叶中绿原酸和沼菊素进行含量测定。方法：采用Kromasil C18色谱柱（4.6mm×250mm,5μm）,流动相乙腈-0.4%磷酸水,梯度洗脱（0-60min,5%-85%乙腈）,流速1.0mL·min-1,检测波长0-18min,327nm,18.1-60min,210nm;进样量10μL,柱温30℃。结果：建立不同大小亚贡叶指纹图谱,较大的亚贡叶样品绿原酸含量高（1.5882mg·g-1）,较小的亚贡叶样品沼菊素含量高（5.6374mg·g-1）。结论：亚贡叶样品中绿原酸、沼菊素含量与叶子大小有相关性。该方法操作简便,结果准确,重复性好,实验结果可用于指导亚贡叶的质量控制。

Objective： To establish an HPLC fingerprint of different size of yacon leaves,and determine the content of chlorogenic acid and enhydrin.Method： The fingerprints were obtained on a Kromasil C18（4.6mm × 250mm,5μm） with the gradient elution solvent system composed of acetonitrile and 0.4% phosphoric acid.The flow rate was 1.0mL·min-1.The column temperature was maintained at 30 ℃,and the detection wavelength was 0-18min,327nm,18.1-60min, 210nm.Result： The fingerprint of yacon leaves was established.Larger leaves samples with high content of chlorogenic acid（1.588 2mg·g-1）,and the small yacon leaves with high content of enhydrin（5.637 4mg·g-1）.Conclusion： The chlorogenic acid and enhydrin content in yacon leaves was correlated with the size.This method is easy to operate with the high exactitude and the excellent repetition for the quantitative analysis of yacon leaves.