扎冲十三味丸抗栓作用及溶栓机制

Antithrombotic Effect and Thrombolysis Mechanism of Zhachong Shisan Wei Pill

目的:研究扎冲十三味丸溶栓作用及机制。方法:大鼠随机分为5组:模型组、扎冲十三味丸高、中、低3个剂量(1.728,0.864,0.432g·kg-1)组、复方丹参滴丸0.12g·kg-1组。运用动静脉环路法测定扎冲十三味丸对大鼠旁路血栓形成的影响;通过在体外血栓的溶解百分率,研究该药体外血栓溶解作用;通过给药后不同时间大鼠纤维蛋白原降解产物(fibrindegradationproducts,FDP),研究该药的体内溶栓作用。结果:与模型组比较,扎冲十三味丸各剂量组,可使血栓质量明显降低(P<0.001),血栓长度明显降低(P<0.05,P<0.001)。模型组与正常对照组比较,血液中的FDP含量有所增加,与模型组比较,扎冲十三味丸中、高剂量组血栓降解产物FDP含量显著升高(P<0.05)。结论:扎冲十三味丸在体内、外有显著的抗栓和溶栓作用,降解纤维蛋白是抗血栓与溶栓的作用机制之一。

Objective： To investigate the antithrombotic effect and the mechanism of the thrombolysis effect of mongolian medicine Zhachong Shisan Wei pill.Method： The rats were randomly divided into 5 groups：control group,Zhachong Shisan Wei pill in high,middle and low dose（1.728,0.864,0.432g·kg-1） groups,and compound Danshen dripping pills treatment group with 0.12g·kg-1.The effects of Zhachong Shisan Wei pill on the formation of thrombus in rats were observed through the way of arteriovenous loop.The dissolution percentage of thrombus in vitro was measured.The fibrinogen degradation product（FDP） of rat at different time after administration was studied in vivo.Result： Compared with the model group,Zhachong Shisan Wei pill with different dose could significantly decrease the weight of thrombus（P〈0.001）,the length of thrombus（P〈0.05,P〈0.001）.Model group increased the content of FDP compared with the control group.Compared with model group,effect of Zhachong Shisan Wei pill on the FDP content of the thrombus degradation products was decreased（P〈0.05）.Conclusion： Zhachong Shisan Wei pill have significant antithrombotic and thrombolysis effects in vivo and in vitro.Degradation of fibrin is one of the mechanisms of antithrombotic and thrombolysis.