中药干预糖尿病鼠肾脏蛋白激酶C信号通路的研究进展

Progress of Chinese Medicine on Kidney of Diabetic Rat Protein Kinase C

细胞信号转导系统己成为近年来研究糖尿病肾病(diabetic nephropathy,DN)的热点之一,高糖作为始动因子,诱导蛋白激酶C(protein kinase C,PKC)信号通路激活并促进DN发生、发展的研究倍受关注。诸多研究显示PKC信号通路的异常激活参与了DN的发生发展,且其重要作用已逐渐确立:PKC信号通路激活可明显影响血液动力学、增加血管通透性以及细胞外基质(extracellular matrixc,ECM)沉积、抑制Na+-K+-ATP酶活性和参与细胞增殖等。并且PKC抑制剂的使用为DN防治开辟了新视角与途径。研究证实,单味中药及其提取物(如牛蒡子及其提取物、槲皮素等)及一些中药复方(如菟箭合剂、六味地黄加味胶囊等)对DM鼠肾脏有部分保护作用,对PKC信号通路也有良好的抑制作用。但现有研究证据表明PKC信号通路抑制剂多为非特异性,且副作用大,而特异性PKC信号通路抑制剂尚在研究中,且迄今无成功应用到临床的先例。因此,随着中药现代化研究加快,深入研究中药抑制PKC信号通路激活,对从信号通路干预DN进展具有重要意义。本文就目前中药干预PKC信号通路与DN研究进展综述,为今后用中药作为PKC抑制剂来防治DN提供一定实验与理论依据。

Cell signal transduction system has become one of the hot research of diabetic nephropathy （DN） in recent years, as the initiating factor induced by high glucose, protein kinase C （PKC） signaling pathway activation and promote the research development of DN occurred, people pay more attention to the. Many studies show that the abnormal activation of PKC signaling pathway is involved in the occurrence and development of DN, and its important role has gradually established： activation of PKC signal pathway can significantly affect the hemodynamics, increased vascular permeability and extracellular matrix deposition, inhibiting the activity of Na ＋- K ＋-ATP and involved in cell proliferation. Opened up a new perspective and approach and the use of PKC inhibitors for the prevention and control of DN. The study confirmed that, Chinese herbs and their extracts, and some traditional Chinese medicine compound has protective effect on DM of rat kidney, also have good inhibitory effect on PKC signal pathway. But the existing research evidence that PKC signaling pathway inhibitors are usually nonspecific, and the side effect is big, but the specificity of PKC signaling pathway inhibitor is still in study, and so far no successful application to clinical precedent. Therefore, with the modernization of traditional Chinese medicine to accelerate, in-depth study of traditional Chinese medicine inhibits the activation of the PKC signaling pathway, has the important meaning to the signal path from intervention in the progress of DN. This paper will present the intervention of traditional Chinese medicine progress in PKC signaling pathway and DN research, for the future use of traditional Chinese medicine as PKC inhibitor for the prevention of DN and provide experimental and theoretical basis.