续骨酒质量标准研究

Quality Standard Research for Xugujiu

[目的]建立续骨酒的定量定性检测方法,为续骨酒质量控制提供理论依据.[方法]采用薄层色谱法（TLC）对续骨酒中的栀子、地黄定性鉴别;采用高效液相色谱法（HPLC）测定栀子苷的含量.[结果]TLC鉴别分离度好、专属性强;含量测定中栀子苷在0.151 1～2.518 0 mg范围内呈良好线性关系,线性回归方程为y=925 558X-11 386,r =0.999 9（n =7）,平均回收率为99.4％,RSD％为1.4.[结论]该试验所建立的方法简便、准确、专属性强、精密度高、重复性好,可作为续骨酒的质量控制方法.

[ Objective ] To establish qualitative and quantitative detection method for Xugujiu, and provide a theoretical basis for the quality con- trol. [ Method] Gardeniae Fructus and Rehmanniae Radix were identified by TLC, the content of Jasminoidin was determined by HPLC. [ Re- sult ] The spots in TLC was clear, the negative control was no interference. Content determination of Jasminoidin had good linear relationship be- tween 0. 151 1 to 2. 518 mg, the linear regression equation was Y=925 558X- 11 386,r =0. 999 9（n =7） , the average recovery was 99.4% , RSD 1.4%. [ Conclusion] The method is simple, accurate, specified, high precision and good repeatability. It can be used for quality control of Xugujiu.