液相色谱法测定辣椒中克百威农药残留量的不确定度评定

Evaluation of Measurement Uncertainty in Determination of Carbofuran Residue in Chilli by Liquid Chromatography

[目的]了解测定蔬菜中氨基甲酸酯类农药多残留量不确定度的主要来源,通过一些方法对不确定度贡献度大的进行改善修正,从而进一步提升检测结果的可靠性。[方法]对辣椒中克百威农药残留的不确定度进行评定和分析,找出影响不确定度的因素,对各个不确定度分量进行评估并比较各分量对总不确定度的贡献,从而给出测定结果的置信区间。[结果]分析得出影响结果的主要不确定度分量是色谱仪峰面积的测定和总重复性。[结论]在检测过程中,需在检测前做好仪器平衡和调试,确保仪器处于良好的工作状态,并在重复检测时尽可能排除其他干扰。

[ Objective] To find out the main sources of measurement uncertainty in the data about determination earbamate pesticides in vege- tables, through some methods to improve and correct the larger contribution of the uncertainty, thus further improve the reliability of detection results. [ Method] Find out the influencing factors of uncertainty by evaluation of measurement uncertainty in determination of carbofuran resi- due in chilli, then evaluate each component of uncertainty and compare each of the components on contribution of the total uncertainty, thus ob- tain the determination results of the confidence interval. [ Result] It was found that the results of the main components of the uncertainty is the chromatograph peak area determination and total repeatability. [ Conclusion] It＇s necessary that balance and debug the instrument before the detection, ensure the instrument is ready, and as much as to exclude other interference in duolieate detection.