猪弓形虫病的实验室诊断与治疗

The Laboratory Diagnosis and Therapy of Swine Toxoplasmosis

[目的]对猪场发生弓形虫的病例进行诊断和治疗,并提出防治措施。[方法]通过猪的临床检查、脏器的剖检变化观察、病料的染色镜检和PCR方法对疑似弓形虫病进行诊断。对病猪采用复方磺胺嘧啶钠治疗,对全群猪口服磺胺脒预防。[结果]通过病猪的临床症状、剖检变化确定为弓形虫病,染色镜检能观察到虫体,通过PCR扩增出病猪脏器中弓形虫的特性条带。[结论]复方磺胺嘧啶钠能控制该猪场弓形虫病的病情。

[Objective] The aim was to diagnose and remedy swine toxoplasmosis, and put forward the prevention and treatment measures. [ Method ] The suspected swine toxoplasmosis was diagnosed by clinical examination, pathological lesion, and latratory tests. The sick pigs trea- ted with the compound sulfamonomethoxine sodium, and the other pigs oral sulfaguanidine to prevent toxoplasmosis. [ Result ] The clinical exmni- nation and pathological lesion of sick pigs was according with infected pigs of toxoplasmosis. The specific： repetitive 529 bp DNA fragment for Tox- uplasma gondii was amplified from the suspected tissues genomie DNA by PCR. [ Conclusion] The compound sulfamonomethoxine sodium can control the tnxoplasmosis in the pig farm.