猪O型口蹄疫疫苗免疫抗体消长动态研究

Dynamic Analysis of Immune Antibodies of Type O of Porcine Foot-and-mouth Disease Vaccine

[目的]掌握猪O型口蹄疫疫苗免疫后的抗体消长规律。[方法]应用猪O型口蹄疫灭活疫苗1、灭活疫苗2和合成肽疫苗对198头试验猪进行至少2次免疫,定期采血后用液相阻断ELISA检测方法测定免疫前后抗体效价。[结果]在首次免疫时,疫苗免疫效果受母源抗体的干扰较大;仔猪首免后抗体很难达到完全保护(大于6log2)水平,应适当缩短首免与二免的间隔时间,尽量减少免疫空白期;合成肽疫苗的首免效果较灭活疫苗好,首次免疫推荐使用合成肽疫苗;如果生猪饲养周期过长,免疫2次的生猪出栏时抗体不能达到完全保护水平;规模场试验猪的母源抗体水平低于散养户,随着试验猪日龄的增加和免疫的加强,规模场试验猪的免疫抗体滴度逐渐高于散养户。[结论]该研究可为研究猪O型口蹄疫抗体变化和制定猪O型口蹄疫疫苗的免疫程序提供参考和借鉴。

[ Objective ] The research aimed to grasp the dynamic change laws of antibody after the vaccine immunization of porcine type O foot - and - mouth disease. [ Method] 198 test pigs were immunized for at least twice with inactivated vaccine, inactivated vaccine 2 and syn- thetic peptide vaccine of porcine type O FMD vaccine. After routine sampling, the immune antibody titer was determined by using liquid - phase blocking ELISA. [Result] In the first immunization, the interference of maternal antibody on the immune effect of the vaccine was lar- ger. The antibody of piglets after the first immunization was difficult to achieve complete protection （ more than 61og2）. So the interval time between the first immunization and the second immunization should be reduced and the immune blank period should be reduced as possible. The first immunization effect of the synthetic peptide vaccine was better than that of inactivated vaccines. So the synthetic peptide vaccine was recommended to be used in the first immunization. If the breeding period of pigs was too long, the antibody level of marketing pigs immunized for twice couldn＇ t reach the complete protection level. The maternal antibody level of test pigs in large - scale pig farm was lower than that in scatter - raised farm. With the increasing age and immunization procedure of pigs, the immune antibody titer of test pigs in large - scale pig farm was gradually higher than that in scatter - raised farm. [ Conclusion ] The research could provide references for studying the antibody changes of porcine type O FMD and establishing the vaccine immunization program of porcine type O FMD.