摘要
目的研究芍药总苷(TGP)脂质体的最佳制备工艺及其体外释放情况。方法采用逆向蒸发法制备TGP脂质体,以包封率为评价指标,采用Box-Behnken Design-响应面优化法(BBD-RSM)优化最佳制备工艺参数,并对其体外释放情况进行考察。结果最佳处方为胆固醇与磷脂质量比为1∶2.7,TGP与脂类质量比为1∶29,水化介质用量5.1 m L,药物的平均包封率为73.0%,体外12 h累积释放率为42.18%。结论 TGP脂质体制备工艺合理可行,包封率高,稳定性好,有良好的缓释作用。
Objective To optimize the preparation technology for total glucosides from Paeonia lactiJlora (TGP) liposome and study on its in vitro release. Methods Liposomes were prepared by reverse-phase evaporation technique. Taking the entrapment efficiency (EE) as indexes, the preparation of liposome was optimized using Box-Behnken design-response surface method (BBD-RSM), and the in vitro release was studied. Results The optimal formulation was cholesterol-lecithin (1 :2.7), TGP-lipid (1 : 29), and hydrated medium of 5.1 mL. The average EE were 73.0% and the 12 h in vitro accumulative release rate was 42.18%. Conclusion The optimized process is rational and feasible, and the TGP liposome has the higher EE and good stability with better sustained release in vitro.
出处
《中草药》
CAS
CSCD
北大核心
2015年第3期359-364,共6页
Chinese Traditional and Herbal Drugs
基金
泸州科技局(2012-S-36)
泸州医学院大学生科研项目(201387)