摘要
目的明确雷丸菌丝生长的影响因素,为人工栽培提供依据。方法在不同温度、碳源、氮源、p H、培养基质等培养条件下测定雷丸菌丝日生长量。结果 25℃、p H 6.0~7.0条件下菌丝生长快;1%~3%的碳源,菌丝生长速率差异不显著;蛋白胨、酵母粉等氮源对菌丝生长有抑制作用,玉米粉无抑制;竹叶为雷丸栽培种较适宜的栽培主料,玉米粉的促生长作用明显。结论温度、氮源和基质对雷丸菌丝生长的影响较明显。
Objective To confirm the influential factors on mycelial growth of Omphalia lapidescens and to provide a basis for the artificial cultivation. Methods The mycelial growth rates of O. lapidescens under different culture conditions were measured. Results With the temperature of 25℃ and pH 6.0-7.0, the mycelium of O. lapidescens was in its rapid growth. And there was no significant difference in mycelial growth rate with the concentration of carbon sources between 1% and 3%. Nitrogen sources such as peptone and yeast powder inhibited the mycelial growth, while the corn flour was not inhibited. The more suitable ingredient for the cultivation of O. lapidescens was bamboo leaves, meanwhile the growth-promoting effect of corn flour was obvious. Conclusion The effects of temperature, nitrogen source, and matrix on mycelial growth of O. lapidescens are significant.
出处
《中草药》
CAS
CSCD
北大核心
2015年第3期424-427,共4页
Chinese Traditional and Herbal Drugs
基金
重庆市卫生局中医药科技项目(ZY20132048)
关键词
雷丸
温度
氮源
竹叶
生长速率
Omphalia lapidescens Schroet.
temperature
nitrogen source
bamboo leaves
growth rate
