青龙衣正丁醇部位化学成分研究

Studies on chemical constituents in n-butanol extracts from epicarp of green fruit of Juglans mandshurica

目的研究青龙衣（核桃楸Juglans mandshurica的未成熟的外果皮）正丁醇部位的化学成分。方法采用正、反相硅胶柱色谱,Sephadex LH-20,高效液相制备色谱等方法进行分离纯化,并通过波谱数据分析鉴定化合物的结构。结果从青龙衣正丁醇提取部位中分离得到14个化合物,分别鉴定为5-O-咖啡酰基奎宁酸丁酯（1）、3,5-二咖啡酰奎宁酸丁酯（2）、香草酸-4-O-β-D-（6′-O-没食子酰基）葡萄糖苷（3）、4-羟基-2,6-二甲氧基苯酚-1-O-β-D-吡喃葡萄糖苷（4）、4,5,8-三羟基-α-萘酮-5-O-β-D-吡喃葡萄糖苷（5）、1,4,8-三羟基萘-1-O-β-D-吡喃葡萄糖苷（6）、1,4,5-三羟基萘-1,4-二-O-β-D-吡喃葡萄糖苷（7）、山柰酚-3-O-β-D-吡喃葡萄糖苷（8）、槲皮素-3-O-β-D-吡喃葡萄糖苷（9）、槲皮苷（10）、杨梅苷（11）、阿福豆苷（12）、金丝桃苷（13）、胡萝卜苷（14）。结论化合物1~4为首次从胡桃属植物中分离得到,化合物5、8~12为首次从青龙衣中分离得到。

Objective To study the chemical constituents in n-butanol extracts from the epicarp of green fruits of Juglans mandshurica. Methods The compounds were isolated and purified by silica gel, ODS, Sephadex LH-20 columns, high preparative performance liquid chromatography, etc. And their structures were identified by spectral analysis. Results Fourteen compounds were obtained and identified as 5-O-cafffeoyl quinic acid butyl ester（1）, 3,5-di-O-caffeoylquinic acid butyl ester（2）, vanillic acid-4-O-β-D-（6′-O-galloyl） glucopyranoside（3）, 4-hydroxy-2,6-dimethoxyphenol-1-O-β-D-glucopyranoside（4）, 4,5,8-trihydroxy-α-tetralone-5-O-β-D-glucopyranoside（5）, 1,4,8-trihydroxynaphthalene-1-O-β-D-glucopyranoside（6）, 1,4,5-trihydroxynaphthalene-1,4-di-O-β-D-glucopyranoside（7）, kaempferol-3-O-β-D-glucopyranoside（8）, quercetin-3-O-β-D-glucopyranoside（9）, quercitrin（10）, myricitrin（11）, afzelin（12）, hyperin（13）, and daucosterol（14）. Conclusion Compounds 1—4 are isolated from the plants of Juglans L. for the first time, Compounds 5, 8—12 are isolated from the epicarps of J. mandshurica for the first time.