胶体磨法包合连翘、薄荷混合挥发油的工艺优化研究

Optimization of inclusion process of mixed volatile oil of forsythia oil and peppermint oil by colloid mill method

目的优选胶体磨法制备连翘薄荷油-β-环糊精(β-CD)包合物的最佳工艺。方法以包合物得率和包合率为评价指标,采用单因素试验结合Box-Behnken响应面法,考察β-CD与水的比例、连翘薄荷油与β-CD的投料比和包合时间对包合工艺的影响;通过薄层色谱法、红外光谱法和显微影像初步验证连翘薄荷油-β-CD包合物是否形成。结果β-CD包合连翘薄荷油的最佳工艺为β-CD与水的比例为1∶3,连翘薄荷油与β-CD的比例为1∶9,包合时间为25 min。通过薄层色谱法、红外光谱法和显微影像初步证明了连翘薄荷油包合物的生成。结论胶体磨法包合连翘薄荷油合理可行。

Objective To optimize the preparation of β-cyclodextrin（β-CD） inclusion compound of forsythia oil and peppermint oil using the colloid mill method. Methods With recovery ratio and inclusion rate of volatile oil-β-CD as evaluation indexes, the single factor test combining Box-Behnken response surface methodology was used to study the effects of β-CD-pure water ratio, β-CD-volatile oil ratio, and inclusion time on the inclusion process of volatile oil. Using TLC, IR spectrum, and microscopic image, the inclusion complexes were confirmed. Results The optimum preparation conditions of inclusion compound were as follows： β-CD-pure water ratio was 1∶3, mixture of forsythia oil and peppermint oil-β-CD ratio was 1∶9, and the inclusion time was 25 min. The formation of inclusion complex was preliminary proved by TLC, IR spectrum, and microscopic image. Conclusion The method is reasonable and feasible.