金针菇废弃菌包中纤维素酶浸提工艺优化

Optimization of Extraction Process of Cellulase from Spent Planting Sack of Flammulina velutipes

以金针菇废弃菌包为原料,采用浸提法制备纤维素粗酶液。通过单因素试验和正交试验,分析了浸提时间、p H值、浸提温度和料液比等因素对纤维素酶浸提的影响。结果表明:在最优的浸提温度30℃、p H 5.0、料液比为0.3∶1的条件下浸提1 h,可以浸提出纤维素酶活力为17.01 U/m L的纤维素粗酶液,较工艺优化前提高27.6%。

Cellulase from the spent planting sack of Flammulina velutipes was prepared by extraction. The effects of extraction time,extraction p H- value,extraction temperature and solid- liquid ratio on the activity of cellulase were studied through single-factor tests and orthogonal experiments. The optimum process parameters were obtained as follows： extraction time 1 h,p H- value5. 0,extraction temperature 30 ℃,and solid- liquid ratio 0. 3∶ 1. Under these optimum conditions,the activity of the extracted cellulase was 17. 01 U / m L,which was 27. 6% higher than that before the optimization of extraction process.