伊曲康唑促进结肠癌细胞自噬的作用及机制研究

Itraconazole Promote Autophagy in Colorectal Cancer Cells

目的研究伊曲康唑促进人结肠癌细胞SW480自噬的作用及机制。方法通过MTT法及克隆形成实验检测伊曲康唑对细胞生长增殖的影响,采用免疫荧光及免疫印迹检测伊曲康唑对自噬相关标志蛋白的表达,免疫印迹检测AKT-mTOR信号通路关键蛋白的表达。结果伊曲康唑显著抑制SW480细胞的增殖速率,并且抑制程度呈药物浓度依赖性。免疫荧光和免疫印迹实验结果表明,伊曲康唑促进细胞自噬,并通过调控AKTmTOR信号通路来实现。结论伊曲康挫通过抑制AKT-mTOR信号通路来促进结肠癌细胞自噬。

Objective To study the effect and mechanism in autophagy of Itraconazole in human colon cancer SW480 cells. Methods Detection of itraconazole on growth and proliferation of cells by MTT and cloning formation. Immunofluorescence and immunoblotting were used to detect the expression of marker protein of itraconazole on autophagy,and the expression of key protein of Akt roTOR signaling pathway. Results Itraconazole significantly inhibited cell proliferation in SW480 cells in a concentration dependent manner. Immunofluorescence and Western blotting showed that itraconazole promotes autophagy through AKT-mTOR signal pathway. Conclusion Our result indicated that Itraconazole promote autophagy in colon cancer cells by inhibiting AKT-mTOR signal pathway.