烟草中多元酸和高级脂肪酸的分析

Analysis of Polybasic and Higher Fatty Acids in Tobacco

采用甲酯化、萃取和GC法定量分析了卷烟烟丝中的多元酸如草酸、丙二酸、丁二酸、苹果酸和柠檬酸和高级脂肪酸如月桂酸、肉豆蔻酸、棕榈酸、亚油酸和硬脂酸。分析步骤为 :将含有己二酸 (内标 )的烟末放入硫酸 -甲醇中 ,在室温下酯化 2 2h ,随后用二氯甲烷萃取 3次 ,用饱和氯化钠溶液洗涤 ,在无水硫酸钠上干燥 ,最后进行气相色谱分析。测定了 11种牌号卷烟的有机酸 ,并进行了比较。分析结果表明 :①在所测定的卷烟中 ,所有牌号卷烟烟丝中的苹果酸、草酸、柠檬酸和亚油酸的含量均远远高于其它有机酸的含量 ;②混合型卷烟中柠檬酸和草酸的含量明显高于烤烟型卷烟 ;③混合型卷烟中的棕榈酸和亚油酸含量低于烤烟型卷烟。

The polybasic and higher fatty acids,such as oxalic, malic, citric, palmitic, linoleic and stearic acids in cut tobacco of cigarettes were quantitatively analyzed with methyl esterification, extraction and GC. Tobacco powder containing adipic acid (internal standard) was esterified in H\-2SO\-4\|CH\-3OH at room temperature for 22 hrs, followed by extraction with CH\-2Cl\-2 for 3 times, washed with aqueous saturated NaCl solution, dried over anhydrous Na\-2SO\-4, and determined by GC. The organic acids in cut tobacco of 11 brands of cigarette were determined and compared. The results showed that 1) the content of malic, oxalic, citric and linoleic acids in cut tobacco of all the brands of cigarette tested was much higher than that of their other organic acids, 2) the concentration of citric and oxalic acids in the blended cigarettes was significantly grearer than that in the flue\|cured cigarettes, and 3) the level of palmitic and linoleic acids in the blended cigarettes was lower than that in the flue\|cured cigarettes.