二代测序技术在水稻基因组学和转录组学研究中的应用

Application of the Second Generation Sequencing Technology in Rice Genomics and Transcriptomics

二代测序技术是测序技术的一次革命性突破,它具有高通量、高精度、低成本的优势,为水稻基因组学研究提供了新的研究方法和解决方案。概述了以使用合成法测序和连接法测序为原理的二代测序技术的特点和发展,并总结了其在水稻基因组学,包括结构基因组学和功能基因组学以及转录组学研究中的应用,并就当前测序技术的发展趋势作了展望。

The second generation sequencing technology made a revolutionary breakthrough in the field of sequencing technology with advantages,including high throughput,high precision and low cost.It provided a new method for the research in rice genomics.Here,we reviewed the theory and development of the second generation sequencing technology including sequencing by synthesis and sequencing by ligation,and summarized its applications in genomics,involving structural genomics and functional genomics,and transcriptomics of rice.Finally,the prospects of current sequencing technologies and their applications in rice research were also discussed.