复方金铃四逆四物失笑散对小鼠急性毒性实验研究

Experimental Study on Acute Toxicity of Formula Jinlingsinisiwushixiao Powder on Mice

目的:研究复方金铃四逆四物失笑散对小鼠的急性毒性,为临床安全用药提供科学依据。方法:小鼠单次灌胃给药进行急性毒性预实验,确定半数致死剂量(LD50)。采用24h内以药液最大浓度和最大给药容积灌胃小鼠3次,连续14天观察受试药物对小鼠指标影响,测定最大给药量。结果:小鼠1天内最大给药量为192g(生药)/kg,相当于成人临床推荐日用量的217.4倍。结论:复方金铃四逆四物失笑散对小鼠无明显毒性反应,临床常用剂量安全性高。

Objective：The acute toxicity study of Formula Jinlingsinisiwushixiao Powder on mice was investigated to guide the clinical applications.Methods：The median lethal doses（LD50）was determined by the pre-experiment of acute toxicity with single taking gavage different dosage to the mice.To measure the maximum dosage,the mice were given the highest concentration and largest volume drug 3times a day,and were observed for successive 14 days.Results：The maximum dosage was 192g（crude drug）/kg,which is equivalent to 217.4times of the clinical dose,and did not cause any acute toxicity.Conclusion：Formula Jinlingsinisiwushixiao Powder is of low toxicity,which are relatively safe and reliable to be taken at the prescribed dosage.