miRNA-29b在前列腺癌中的表达及意义

Effects of miRNA-29b on Cell Biological Properties in Prostate Cancer

目的探讨miRNA‐29b（miR‐29b）在前列腺癌中的表达及意义。方法采用荧光实时定量PCR技术（QRT‐PCR）检测前列腺癌、癌旁非肿瘤组织中miR‐29b的表达，采用M T T法检测miR‐29b的上调对细胞增殖及顺铂化疗作用的影响，用流式细胞术检测细胞周期变化，AV／PI双标法FCM检测细胞的凋亡情况， T ransw ell细胞侵袭实验观察细胞侵袭能力。结果 miR‐29b在前列腺癌组织中的表达明显低于癌旁组织。与阴性对照组相比，miR‐29b模拟物组细胞存活率下降，并且增加顺铂对前列腺癌细胞的抑制作用；miR‐29b模拟物转染组亚G0／G1期明显增加，总凋亡率明显下降，侵袭细胞数量明显减少，差异均有统计学意义（ P ＜0．05）。结论 miR‐29b在前列腺癌中表达下降，增加其表达可以抑制前列腺癌细胞的增殖和侵袭能力，促进癌细胞凋亡并且增加癌细胞对顺铂化疗作用的敏感性。

[Objective]To explore the expression of miRNA‐29b （miR‐29b） ,examine its effects on cell bi‐ological properties in prostate cancer （Pca） and discuss its potential clinical values .[Methods]The expressions of miR‐29b in prostate cancer tissues and matched adjacent non‐tumor tissues were detected by real‐time quan‐titative polymerase chain reaction （QRT‐PCR） .The cellular growth and chemotherapeutic effect of cisplatin were measured by methyl thiazol tetrazolium （MTT） assay .Flow cytometry was used to determine the cell cy‐cle changes .And AV/PI double labeling method was utilized to detect the apoptosis of LNCap .Finally the im‐pact of miR‐29b on the invasiveness of LNCap cell was determined by Transwell invasion assay .[Results]The expression of miR‐29b was down‐regulated in Pca tissues versus matched adjacent non‐tumor tissues .Com‐pared with negative controls ,group of transfected miR‐29b showed lower tumor cell survival rate .Moreover , miR‐29b could increase the inhibition of cisplatin on LNCap with much lower cell survival rate .LNCap with transfected miR‐29b revealed a higher percentage of cells during sub‐G0/G1 phase and significant decreases of apoptosis cell rate and invasive cell rate （ P 〈0 .05） .[Conclusion]The expression of miR‐29b is down‐regula‐ted in prostate cancer .A higher expression of miR‐29b inhibits cell proliferation and invasion and induces cell apoptosis in prostate cancer .And the chemosensitivity of cisplatin increases in Pca cell .