一株异养硝化真菌的筛选及其特性

Isolation and Characteristic of a New Heterotrophic Nitrification Fungus

从焦化废水曝气池的活性污泥中，分离纯化出一株高效降解氨氮的异养硝化真菌。通过26SrRNA基因序列分析，再结合菌落形态、生理生化特征，鉴定该菌为青霉属（Penicillium），命名为L1。对该菌的培养基进行优化，选择出最佳的碳氮源、碳氮摩尔比、初始pH值；采用优化培养基培养L1，36h后可将初始质量浓度为130mg／L的氨氮降解至5．61mg／L，降解率为95．68％，且几乎没有亚硝酸盐氮和硝酸盐氮的积累。培养基中同时含有100mg／L氨氮和200-1000mg／L苯胺时，L1对两者同时降解的效果也较好。

A heterotrophic nitrification fungus with high efficiency of removing NH^＋4-N, was isolated and purified from the activated sludge of coking wastewater. By 26S rRNA gene sequence analysis, and combined with colony morphology, physiological and biochemical characteristics, it was identified as Penicillium fungus and named L1. The culture medium was optimized to select the optimum source of carbon and nitrogen,n（C）/n（N） and initial pH. In the optimized medium with initial concentration of 130 mg/L ammonia, L1 degraded the ammonia to 5.61 mg/L after 36 h, the degradation rate was 95.68%,with almost no accumulation of nitrite and nitrate. On the complex medium containing 100 mg/L ammonia and 200-1000 mg/L aniline, L1 degraded both of them with a good effect.