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心肌缺血/再灌注损伤大鼠心肌组织PGC-1α的表达变化及意义

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摘要 目的观察心肌缺血/再灌注损伤大鼠心肌组织过氧化物酶体增殖物受体γ(PPARγ)辅助激活因子1α(PGC-1α)的表达变化,并探讨其意义。方法将21只Wistar大鼠随机分为缺血/再灌注模型组(I/R组)、GW9662组、假手术组,各7例。I/R组采用在体结扎左前降支方法建立缺血/再灌注大鼠模型,结扎左前降支使其缺血30 min,再灌注120 min;GW9662组建立模型1 d前静脉注射GW9662,剂量0.3 mg/kg;假手术组开胸绕过左前降支不做冠状动脉结扎,剩余步骤同I/R组。RT-PCR法检测各组大鼠心肌组织PGC-1αmRNA,Western blotting法检测各组大鼠心肌组织PGC-1α蛋白,ELLSA法测定各组大鼠血清中肌钙蛋白I。结果 I/R组、GW9662组、假手术组PGC-1αmRNA分别为1.70±0.09、1.00±0.07、0.86±0.03,PGC-1α蛋白分别为2.80±0.21、1.00±0.15、0.65±0.05,I/R组、GW9662组与假手术组比较,I/R组与GW9662组比较,P均<0.05。I/R组、GW9662组PGC-1αmRNA及蛋白表达与其血清中c Tn I水平呈负相关(P均<0.05)。结论心肌缺血/再灌注损伤大鼠心肌组织中PGC-1α表达上调,与血清中c Tn I水平呈负相关,具有心脏保护作用。
出处 《山东医药》 CAS 北大核心 2015年第7期29-31,共3页 Shandong Medical Journal
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