抗氧化剂阿魏酸自组装金纳米颗粒的体外抗氧化

In Vitro Antioxidant Activity Study of Ferulic Acid Self-Assembled Gold Nanoparticles

对纳米阿魏酸抗氧化剂在巨噬细胞上的抗氧化保护作用进行检测,并采用电子顺磁-自旋捕获技术和光谱法对纳米阿魏酸在细胞水平上清除自由基的能力进行检测.实验结果表明:与抗氧化剂单体相比,纳米阿魏酸抗氧化剂可以更为有效地清除由细胞外界叔丁基过氧化物(t-BuOOH)刺激所产生的活性氧自由基.同时,通过光谱法对细胞膜脂质过氧化物指标丙二醛进行检测,证明纳米抗氧化剂可以更为有效地保护经受外界刺激的巨噬细胞.通过自组装方式可将抗氧化剂纳米化有利于提高抗氧化剂的抗氧化水平.

The antioxidant protection effects of ferulic acid nanoantioxidant on macrophage cells were studied and the free radical-inhibiting activities of ferulic acid nanoantioxidant were determined at the cellular level using electron spin reso-nance-spin trapping and UV-spectrum method.The results illustrates that the nanoantioxidant could eliminate the reactive oxygen species stimulated by t-BuOOH in cells more effectively than that of antioxidants monomers.Meanwhile,the lipid peroxide detection of malondialdehyde by spectrum method also proved that the nanoantioxidant have a high antioxidant activity on t-BuOOH stimulated macrophage cells.Therefore,it could be concluded that the self-assembled nanoantioxi-dant have a potential for the enhancement of antioxidant activity.