摘要
建立了固相萃取-HPLC法同时测定滇龙胆中抗老年痴呆的7种主要成分gentiside G,gentiside H,gentiside I,gentiside A,gentiside J,gentiside B,gentiside K的含量的方法。滇龙胆样品经乙醇提取环己烷萃取后,用硅胶固相萃取小柱净化,HPLC-DAD对7种苯甲酸酯进行定量检测。7种苯甲酸酯类化合物在一定范围内线性关系良好,相关系数均大于0.999,加样回收率为92.8%~104.6%,RSD为0.8%~4.7%。不同产地龙胆样品中云南产滇龙胆苯甲酸酯含量高于其他产地龙胆,7种化合物中,gentiside B含量最高达0.3425μg/g,gentiside G含量最低只有8.5μg/g。方法为滇龙胆药材的质量控制和资源开发利用提供科学依据。
A solid-phase extraction( SPE) – high performance liquid chromatography( HPLC) method was developed for the simultaneous determination of seven benzoate compounds( gentiside G,gentiside H,gentiside I,gentiside A,gentiside J,gentiside B,gentiside K) of anti-dementia in Gentiana rigescens Franch. The sample was extracted by ethanol and cyclohexane,purified through a SI solid-phase extraction column,and detected by HPLC-DAD. All of benzoate compounds showed good linearities within the certain ranges( r 〉 0. 999). The recoveries ranged from 92. 8% to 104. 6%,with the RSDs of 0. 8% ~ 4. 7%. The contents of seven benzoate compounds in Gentiana rigescens Franch native to Yunnan are higher than those from other regions. The highest content is gentiside B,up to 0. 3425 mg / g,while the lowest content is gentiside G,only 8. 5 μg / g. This method can be used for the determination of benzoate compounds,and provides scientific basis of Gentiana rigescens Franch for the quality control and further application.
出处
《分析试验室》
CAS
CSCD
北大核心
2015年第3期259-262,共4页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金(61280806)
云南省自然科学基金(2013FZ151)
云南省科技计划项目(2012AE002)资助
关键词
滇龙胆
苯甲酸酯
固相萃取
HPLC
老年痴呆
Gentiana rigescens franch
Benzoate
Solid phase extraction
HPLC
Dementia