基于碳球的乙酰胆碱酯酶传感器用于对硫磷的检测研究

Preparation of acetylcholinesterase biosensor based on carbon spheres for parathion detection

以硼掺杂金刚石电极（BDD）为工作电极,利用碳球（Cs）固定乙酰胆碱酯酶（ACh E）,制备ACh E/Cs/BDD传感器,将其用于农药残留对硫磷的检测。在1.0×10-9~2.0×10-7g/L范围内,对硫磷标液的抑制率与其浓度的负对数呈良好的线性关系,线性方程为A（%）=-11.957x＋147.575（%）,相关系数R2为0.999。按照抑制率为10%计算,检出限为0.310×10-11g/L。用该传感器对青菜叶汁进行检测,回收率在89.4%~107.5%范围内。

In this work,the boron-doped diamond（ BDD） electrode was employed as the working electrode,the carbon spheres（ Cs） was used to immobilize acetylcholinesterase（ ACh E） to fabricate the ACh E / Cs / BDD biosensor,which was used to detect parathion. The inhibition rate versus the logarithm of parathion are linear in the range of 1. 0 × 10-9to 2. 0 × 10-7g / L,with a linear equation of A（ %） =-11. 957 x ＋ 147. 575（ %）,and the correlation coefficient（ R2） is 0. 999. Calculated by inhibition rate of 10%,the detection limit of parathion is 0. 310 × 10-11 g / L. The sensor could be used for detecting cabbage leaf juice samples,with recoveries of 89. 4% ~ 107. 5%.