醌亚胺类碱性染料荧光猝灭法测定肝素钠

Determination of heparin with quinoneimine basic dyes by fluorescence quenching method

建立了醌亚胺类碱性染料（藏红O、中性红、耐尔蓝A）荧光猝灭法测定肝素钠的方法。方法的线性范围和检出限分别为0.008~0.8 mg/L,3μg/L（藏红O体系）;0.06~2.0 mg/L,40μg/L（中性红体系）;0.05~1.0 mg/L,20μg/L（耐尔蓝A体系）,其中以藏红O体系最为灵敏。以藏红O体系为例研究了共存物质的影响,方法已用于肝素钠注射液效价的测定。

Based on notable flurescence quenching effect of quinoneimine basic dyes（ Safranine O,neutral red,Nile Blue A）,a sensitive fluorescence quenching method was developed for the determination of heparin. The fluorescence quenched intensity of quinoneimine basic dyes was linear with heparin in a certain concentration range. Under the optimum conditions,the linear ranges of heparin were 0. 008 ~ 0. 8 mg / L for Safranine O system,0. 06 ~ 2. 0 mg / L for neutral red system and 0. 05-1. 0 mg / L for Nile Blue A system. The detection limits were 3. 0 μg / L for Safranine O systems,40 μg / L for neutral red system and 0. 02 mg / L for Nile Blue A system. Taking Safranine O system as an example,the effect of coexisting substances was investigated. The method is sensitive and selective,and it can be applied to determination of the potency of heparin sodium injection with satisfactory results.