鸡可食性组织中癸氧喹酯残留的HPLC检测方法

Determination of decoquinate residues in chicken edible tissues by high performance liquid chromatography

采用高效液相色谱法测定鸡可食性组织中癸氧喹酯残留量。鸡可食性组织中癸氧喹酯残留经乙腈和乙酸乙酯提取后,采用C18色谱柱直接分离测定。流动相为乙腈、甲醇、水(体积比为50:25:25,加入3.4 g·L^(-1)氯化镁)溶液,紫外线检测波长为330 nm。结果表明:外标法定量测定癸氧喹酯对照品溶液为0.5~5 mg·L^(-1)时呈良好的线性相关(r>0.999 7);鸡空白组织样品在0.5、1、5 mg·kg^(-1)添加水平上,癸氧喹酯在鸡可食性组织(肌肉、肝脏和肾脏)中回收率为70%~100%,日内和日间变异系数均小于10%。方法检出限和定量限分别为0.1和0.2 mg·kg^(-1)。该方法适用于鸡可食性组织中癸氧喹酯残留量的测定。

A method was developed for the determination of decoquinate in chicken tissues by high-performance liquid chromatography（HPLC）.The decoquinate residues in chicken tissues were extracted with a mixture of acetonitrile and ethylacetate.The extracts were determined by HPLC on C18 column with ultraviolet detection at 330 nm,and mobile phase was acetonitrile-methanol-water（Volume ratio 50：25：25） containing 3.4 g·L^-1 MgCl2.Under above chromatography conditions,the response for decoquinate was linear in the range of 0.5-5 mg·L^-1（r0.999 7）.Recoveries at fortified level of 0.5,1 and 5 mg·kg^-1 in chicken edible tissues（muscle,liver and kidney） ranged from 70%—100%and both the within-day coefficient variation（C.V） and between-day C.V were less than 10%.The detection and quantity limits were 0.1 and 0.2 mg·kg^-1,respectively.This method could meet the demand of residue determination of decoquinate in chicken edible tissues.