摘要
模拟人体生理条件(pH=7.4)下,用伏安法、荧光、紫外和圆二色谱法研究沙丁胺醇(SAL)与牛血清白蛋白(BSA)的相互作用。用Stern-Volmer和双对数方程处理荧光数据,得到沙丁胺醇与BSA的结合常数为3.12×106L·mol-1、结合位点数约为1。循伏安法确定了SAL与BSA的结合比,并计算了结合常数,结果与用双对数方程计算得到的常数吻合。用紫外和圆二色谱验证了BSA与沙丁胺醇作用时构象的改变。
Under the imitated physiological conditions( pH = 7. 4),the interaction between salbutemol( SAL) and bovine serum albumin( BSA) was investigated by the fluorescence,UV-vis spectroscopy,voltammetry and circular dichroism. Voltammetric results indicated the formation of the SAL2-BSA complex. Experimental data were processed with Stern-volmer plot and Line weaver-Burk equation. The way of fluorescence quenching was static quenching,and then calculated the binding constant( 3. 12 10^6L·mol^-1) and binding sites( -1) and the thermodynamics parameters. The changes in the secondary structure of BSA after its binding with the ligand were studies with UV-vis and circular dichroism spectroscopy. The secondary structure alteration of BSA in the presence of salbutemol were observed.
出处
《南昌大学学报(理科版)》
CAS
北大核心
2014年第1期69-73,共5页
Journal of Nanchang University(Natural Science)
基金
国家自然科学基金资助项目(21065007)
南昌大学食品科学与技术国家重点实验室基金资助(SKLF-ZZA-201302
SKLF-ZZB-2013003
SKLF-KF-201004)
关键词
光谱法
伏安法
圆二色谱
沙丁胺醇
牛血清白蛋白
相互作用
Spectro scopy
Voltammetry
Circular dichroism
Salbutemol
Bovine serum albumin
Interaction