共轭亚油酸异构体对胰岛素抵抗脂肪细胞消耗葡萄糖的影响

Effects of conjugated linoleic acid isomers on glucose consumption of insulin-resistant adipocytes

为了解天然食物中主要存在的2种共轭亚油酸异构体(c9,t11-CLA和t10,c12-CLA)改善胰岛素抵抗的活性,建立胰岛素抵抗3T3-L1脂肪细胞模型,分别以5,10,20μmol·L-1的c9,t11-CLA或t10,c12-CLA进行干预处理,测定干预处理后培养液中葡萄糖浓度和细胞中脂肪含量的变化。通过考察空白组(CON)、模型组(MOD)和罗格列酮阳性对照组(ROS),结果发现c9,t11-CLA不能增加胰岛素抵抗脂肪细胞的葡萄糖消耗量,而t10,c12-CLA能增加胰岛素抵抗脂肪细胞的葡萄糖消耗量,并且t10,c12-CLA的作用浓度和时间与葡萄糖的消耗量呈一定的量效关系。20μmol·L-1t10,c12-CLA处理组的葡萄糖消耗量和ROS相当(P>0.05),但前者细胞中的脂肪含量显著低于后者(P<0.05)。显示t10,c12-CLA改善胰岛素抵抗3T3-L1脂肪细胞的活性可能与其降低细胞中的脂肪含量有关。

Two main Conjugated Linoleic Acids（ CLAs） c9,t11 and t10,c12 in natural food were selected to investigate their effects on enhancing insulin sensitivity. The insulin-resistant 3T3-L1 adipocytes were induced by high concentration insulin,which was used as cell model. Glucose consumption was tested each 24 h for all treatments. It was found that c9,t11-CLA did not have any activity in enhancing insulin sensitivity. However,t10,c12-CLA showed significant ability to enhance glucose consumption in a concentration and time dependent manner. For instance,at the concentration of 20 μmol·L^-1,t10,c12-CLA had the same effect as rosiglitazone（ P〉 0. 05）,which was a commonly used medicine in type-2 diabetes. The content of lipid in the cells was significantly decreased by the treatment of t10,c12-CLA（ P〈0. 05）,which might be associated with the activity of enhancing insulin sensitivity.