摘要
目的:探讨髋关节液中Ⅱ型胶原羧基端端肽( CTX-Ⅱ)含量与股骨头坏死软骨病变的关系。方法选择38例(43髋)股骨头坏死和6例(4髋、2膝)阴性对照患者,应用双抗体夹心酶标免疫法测定关节液中CTX-Ⅱ含量,比较各组间的差异及相关性。结果股骨头坏死各组关节液中CTX-Ⅱ的含量明显高于阴性组,ⅢA组与阴性组比较( P=0.109),ⅢB组、ⅢC组分别与阴性组比较(P<0.01),三组间两两比较(P<0.05);<6月组与阴性组比较(P>0.05),6~11月组、≥12月组CTX-Ⅱ含量高于阴性组(P<0.01),<6月组CTX-Ⅱ含量低于6~11月组及≥12月组(P<0.05),而6~11月组与≥12月组比较( P>0.05);病因分组比较无差异( P>0.05);股骨头坏死髋关节液中CTX-Ⅱ含量与塌陷程度、疼痛时间、病因等因素的Spearman秩相关系数分别为0.943、0.595( P<0.01),0.112(P>0.05)。结论股骨头坏死关节液CTX-Ⅱ含量可作为生物标记物反映关节软骨病变程度,为临床制定合理治疗方案提供依据。
Objective To explore the relationship between the level of C-telopeptide of type Ⅱ collagen (CTX-Ⅱ ) in the synovial fluid and the cartilage lesion of osteonecrosis of the femoral head (ONFH). Methods The synovial fluid liquid (2 -5 ml) of 38 ONFH patients (43 hips) and six (four hips, two knees ) negative patients were extracted in the operation. The CTX-Ⅱ in the synovial fluid was measured by the double antibody sandwich enzyme-linked immunosorbent assay. The differences and correlation were compared between the groups. Results The CTX-Ⅱ of the ONFH groups was significantly higher than that of the negative group. There was no significant difference (P 〉 0. 05 ) between the stage m A group and the negative group. The differences among the stage m B group, the stage Ⅲ C group and the negative group were statistically significant by paired comparison ( P 〈 0. 01 ). There was statistically significant difference ( P 〈0. 05 ) between any two groups of stage Ⅲ. There was no significant difference (P 〉0. 05) between the less-than-six-month group and the negative group. The CTX- 1I results in the 6 - 11 months group and the over-12-month group were higher than the negative group, and the difference was statistically significant (P 〈 0. 01 ). The patients in the less-than-six-month group had lower CTX-Ⅱ level than the patients in the 6 - 11 months group and the over-12-month group; the differences were statistically significant ( P 〈 0. 05 ). There was no significant difference ( P 〉 0.05 ) between the 6 - 11 months group and the over-12-month group. As for the etiology groups, there was no statistically significant difference ( P 〉 0. 05 ). The spearman rank correlation coefficients of the CTX- Ⅱ level in the ONFH joint fluid with collapse extent, pain duration and etiology were 0.943, 0.595 (P 〈 0.01), 0.112 (P 〉 0.05), respectively. Conclusion CTX-Ⅱ in the ONFH joint fluid can be used as a biomarker reflected the degree of articular cartilage lesion, and the assessment can provide a guidance for the treatment of ONFH.
出处
《中华关节外科杂志(电子版)》
CAS
2014年第5期43-46,共4页
Chinese Journal of Joint Surgery(Electronic Edition)
基金
国家青年科学基金项目(81302990)
广东省高等学校学科与专业建设专项资金项目(2013LYM 0010)
广东省医学科研基金项目(B2013164)