摘要
A total of 22 mierosatellite DNA markers were selected to analyze the genomic DNA of 176 wild population progenies from 4 families. The normal distri- bution of seven characters （body length, fork length, head length, snout length, mouth length, mouth width and eye diameter） was analyzed using SPSS software. And the results indicated that all the characters had a characteristic of continuous variation which belonged to the typical quantitative characters or polygenie inheri- tance characters, and fitted the normal distribution. Meanwhile, GLM procedure was used to analyze the correlation between the 22 microsatellites and 7 characters ; and multiple comparisons of the markers significantly correlative to these characters were carried out based on the genotypes. Results showed in that among the 22 micrasatellite loci, there were 10 markers significantly correlative to at least one character. The maximum number of markers related to mouth length and eye diame- ter was 7. Results of multiple comparisons showed that there were signifieant differences of genotypes from the same character in every marker. Screening of these markers provided basis for the further study on molecular marker-assisted breeding.
A total of 22 mierosatellite DNA markers were selected to analyze the genomic DNA of 176 wild population progenies from 4 families. The normal distri- bution of seven characters （body length, fork length, head length, snout length, mouth length, mouth width and eye diameter） was analyzed using SPSS software. And the results indicated that all the characters had a characteristic of continuous variation which belonged to the typical quantitative characters or polygenie inheri- tance characters, and fitted the normal distribution. Meanwhile, GLM procedure was used to analyze the correlation between the 22 microsatellites and 7 characters ; and multiple comparisons of the markers significantly correlative to these characters were carried out based on the genotypes. Results showed in that among the 22 micrasatellite loci, there were 10 markers significantly correlative to at least one character. The maximum number of markers related to mouth length and eye diame- ter was 7. Results of multiple comparisons showed that there were signifieant differences of genotypes from the same character in every marker. Screening of these markers provided basis for the further study on molecular marker-assisted breeding.
基金
Supported by the National Science and Technology Support Plan Project(2012BDA25B10)
the Science and Technology Project of Heilongjiang Province(GZ11B201)
the Fundamental Research Fund for Central Public Welfare Research Institutes(201109)
the Open Project of Key Laboratory of Freshwater Aquatic Biotechnology and Breeding,Ministry of Agriculture(2012NYBSJ-03)
