响应面法优化超声波提取蓝莓多酚工艺

Optimization of ultrasonic-assisted extraction process of blueberry polyphenols using response surface methodology

【目的】对超声波提取蓝莓工艺进行优化,为其商业化生产提供技术支持。【方法】以蓝莓冻干粉为试验原料,在单因素试验基础上选择乙醇浓度、料液比、超声时间等3个因素进行响应面设计,采用Box-Behnken中心组合试验设计和响应面分析法,建立以蓝莓多酚含量为响应值的二次回归方程。【结果】通过响应面分析建立蓝莓多酚超声萃取回归方程为:y=10.88-0.15x1+0.042x2+0.055x3-0.27x1x2-0.15x1x3-0.090x2x3-0.56x12-0.35x22-0.15x32(x1为乙醇浓度,x2为料液比,x3为超声时间,y为蓝莓多酚萃取率,R2=0.9692),该模型拟合度好;并确定蓝莓多酚萃取的影响因素顺序为:x1>x3>x2;最佳提取工艺条件为:提取次数两次、超声功率400 W、乙醇浓度55%、料液比1∶25、超声时间1.5 h,在此条件下蓝莓多酚含量为11.05 mg/g。【结论】研究建立的模型适合蓝莓多酚超声波提取,可用于实际生产。

【Objective】Ultrasonic-assisted extraction process of blueberry polyphenols was improved to provide technical support for its commercialized production. 【Method】The freeze-dried powder of blueberries was used as material. Three factors of ethanol concentration, solid/liquid ratio, ultrasonic time of polyphenol extraction were chosen and used in the response surface analysis on the basis of single-factor experiment. The quadratic regression model with the response values of polyphenols yield was established by Box-Behnken design. 【Result】The full model of polyphenol extraction in blueberries was established using response surface methodology, which was y=10.88-0.15x1＋0.042x2＋0.055x3-0.27x1x2-0.15x1x3-0.090x2x3-0.56x1 2-0.35x2 2-0.15x3 2（x1ethanol concentration, x2material/solid, x3ultrasonic time, Y polyphenol yield）. R2 was 0.9692. The degree of fitting of the model was good. The order of impact factors was x1〉x3〉x2. The optimized extraction conditions were as follows： ultrasonic power of 400 W, extraction times of 2, ethanol concentration of 55%, solid/liquid ratio of 1∶25, and ultrasonic time of 1.5 h. The polyphenols yield of 11.05 mg/g was achieved under the optimized conditions. 【Conclusion】This model was suitable for extracting blueberry polyphenols and could be used for practical production.