广西不同产地对叶百部4种生物碱含量测定及止咳活性比较

Comparative Study of Four Alkaloids Contents and Antitussive Activities of Stemona tuberosa from Different Habitats of Guangxi Province

目的：测定广西不同产地对叶百部中4种生物碱的含量,评价对叶百部止咳活性。方法：HPLC含量测定方法采用Merck Purospher STAR RP18（250 mm×4.6 mm,5μm）色谱柱,流动相为0.05%氨水-乙腈,流速：1.0m L/min;检测波长：210 nm;柱温：40℃。采用豚鼠枸橼酸试验测定不同产地对叶百部总生物碱的止咳活性。结果：4种生物碱相关系数均大于0.999,加样回收率98.24%-101.21%。广西产对叶百部以百部新碱为主要成分类型,其次为对叶百部碱和金刚大碱,少见新对叶百部碱。广西产对叶百部总生物碱对豚鼠的止咳活性呈浓度依赖性。结论：广西防城东兴产对叶百部兼具降低咳嗽次数和延长咳嗽潜伏期的功效,防城东兴是目前实验中广西产对叶百部质量较好的地区。

Objective： To compare the contents of four alkaloids and antitussive activities of Stemona tuberosa from different habitats of Guangxi Province. Methods： The HPLC separation was performed on a Merck Purospher STAR RP18（ 250 mm × 4. 6 mm,5 μm）column by gradient elution using 0. 05% ammonia-acetonitrile as the mobile phase. The flow rate was 1. 0 m L / min,the dectection wavelength was set at 210 nm,and the column temperature was 40 ℃. The antitussive potency of total alkaloids of Stemonae Radix from different habitats was evaluated on guinea pigs with citric acid aerosol to induce cough. Results： The range of recoveries of this mehtod was98. 24% - 101. 21%,with all the constituents showing good linearity（ the correlation coefficents above 0. 999）. The major chemotype of Stemonae Radix in Guangxi was stemoninine,following by tuberostemonine and croomine,and finally neotuberostemonine. The antitussive activitiy of Stemona tuberosa was in a concentration-dependent manner. Conclusion： Stemonae Radix from Dongxing,Fangcheng can reduce cough times and prolong cough incubation period,and thus Dongxing,Fangcheng is the best habitat in Guangxi in the present experiments.