硫磺熏蒸党参对小鼠免疫功能的影响

Effect of Codonopsis Radix Maintained with Sulfur Fumigation on Immune Function in Mice

目的:考察硫磺熏蒸党参对小鼠免疫功能的影响。方法:健康小鼠分为硫磺熏蒸党参高、中、低剂量组,低温真空养护党参组及空白对照组。小鼠连续灌胃给药10 d后,进行体质量变化、免疫器官指数、血细胞分析、巨噬细胞吞噬功能、细胞因子IL-2和IFN-γ含量的比较分析。结果:与空白对照组比较,中、高剂量硫磺熏蒸党参的水提液可抑制小鼠体质量增长;硫磺熏蒸党参高剂量组小鼠白细胞数显著增加;各给药组小鼠巨噬细胞吞噬功能显著提升,脾脏指数升高,细胞因子的产生增加,其中硫磺熏蒸党参中剂量组的脾脏指数和IFN-γ与空白对照组比较差异显著。结论:小鼠服用党参水提液对细胞免疫功能有一定促进作用;硫磺熏蒸党参的水提液与低温真空养护党参的水提液在实验期内对免疫功能的影响无统计学差异。然而服用硫磺熏蒸党参对小鼠的食欲和体质量增长会产生负面影响。

Objective： To investigate the immune function of mice being given the extract of Codonopsis Radix maintained with sulfur fumigation. Methods： Mice were divided into five groups. Except the normal control group,the mice were fed with the extract of Codonopsis Radix maintained with sulfur fumigation at the high,medium and low doses,as well as medium dose of Codonopsis Radix maintained with low-temperature vacuum method,respectively. Mice were treated once a day for 10 continuous days. Weight change,organ indexes,blood cell indices,macrophage phagocytic function,and IL-2 and IFN-γ levels were measured. Results： Compared with normal control group,Codonopsis Radix maintained with sulfur fumigation at medium and high doses inhibited body weight increase of mice;white blood cell count of high dose group was significantly increased; significant increase of macrophage phagocytosis were observed for all groups except the normal control group; and spleen index and IFN-γ level of Codonopsis Radix maintained with sulfur fumigation medium dose group were increased significantly. Conclusion： Codonopsis Radix maintained with sulfur fumigation can promote mouse immune function to a certain degree. There was no difference in immune effect between Codonopsis Radix maintained with sulfur fumigation and low-temperature vacuum method during experimental period. However,taking the extract of Codonopsis Radix maintained with sulfur fumigation can exert negative effect on appetite and body weight in mice.