黔产苗药百尾参UHPLC-DAD指纹图谱研究

Study on UHPLC-DAD Fingerprint of Disporum cantoniense

目的：利用UHPLC-DAD建立黔产苗药百尾参的指纹图谱,为评价其质量提供理论依据。方法：以Agilent Eclipse ZORBAX Plus C18（100 mm×2.1 mm,1.8μm）为色谱柱,0.1%甲酸乙腈（A）-0.1%甲酸水（B）为流动相系统梯度洗脱,流速为0.3 m L/min,检测波长290 nm,柱温40℃,进样量3μL。指纹图谱进行了相似度评价和主成分分析。结果：建立了黔产苗药百尾参的UHPLC-DAD指纹图谱,标定了12个共有峰,确认了其中3个成分。30批药材相似度结果为0.766-0.994,主成分分析提示1号峰和12号峰在指纹图谱中具有代表意义。结论：该方法准确、可靠,可用于百尾参药材的鉴别和质量控制。

Objective： To establish an UHPLC-DAD fingerprint of 30 batches of Miao medicine Disporum cantoniense from Guizhou,and to provide a theoretical evidence to evaluate its quality. Methods： The analysis was carried out on an Agilent Eclipse ZORBAX Plus C18（ 100 mm × 2. 1 mm,1. 8 μm） column with a mobile phase consisting of acetonitrile-water（ containing 0. 1% formic acid）with gradient elution,and the flow rate was 0. 3 m L / min. The column temperature was set at 40 ℃ and UV detection wavelength was set at 290 nm. The sample injection volume was 3 μL. The similarity evaluation and principal component analysis（ PCA） of these fingerprints were carried out. Results： The UHPLC-DAD fingerprint was established and compared by 30 batches of samples similarity with 12 common peaks and 3 peaks were identified. The similarities of 30 batches of Disporum cantoniense were between 0. 766 and 0. 994. The principal component analysis showed that compounds 1 and 12 were representative for the fingerprint. Conclusion： The method is accurate and credible,which can be used for identification and quality control of Disporum cantoniense.